8H67

type I-B Cascade bound to a PAM-containing dsDNA target at 3.8 angstrom resolution.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	CRISPR RNA	polymer	71	22646.3	1	GenBank (662706368)	synthetic construct
2	B (C)	Target DNA	polymer	11	3390.3	1		synthetic construct
3	C (D)	Non target DNA	polymer	9	2705.8	1		synthetic construct
4	D (A)	CRISPR associated protein Cas5	polymer	237	26592.4	1	UniProt (A0A068N1Y0)	Synechocystis sp. PCC 6714
5	E, F, G, H, I... (E, F, G, H, J...)	CRISPR associated protein Cas7	polymer	301	33789.1	7	UniProt (A0A068N458)	Synechocystis sp. PCC 6714
6	K (L)	CRISPR associated protein Cas8	polymer	603	69287.3	1	UniProt (A0A068N831)	Synechocystis sp. PCC 6714
7	L, M, N (M, N, O)	CRISPR associated protein Cas11b	polymer	124	14538.5	3	UniProt (A0A068N831)	Synechocystis sp. PCC 6714

Sequence modifications

L: 33 - 551 (UniProt: A0A068N831)

PDB	External Database	Details
Met -51	-	initiating methionine
Gly -50	-	expression tag
Ser -49	-	expression tag
Ser -48	-	expression tag
His -47	-	expression tag
His -46	-	expression tag
His -45	-	expression tag
His -44	-	expression tag
His -43	-	expression tag
His -42	-	expression tag
His -41	-	expression tag
His -40	-	expression tag
Ser -39	-	expression tag
Gln -38	-	expression tag
Trp -37	-	expression tag
Ser -36	-	expression tag
His -35	-	expression tag
Pro -34	-	expression tag
Gln -33	-	expression tag
Phe -32	-	expression tag
Glu -31	-	expression tag
Lys -30	-	expression tag
Gly -29	-	expression tag
Gly -28	-	expression tag
Gly -27	-	expression tag
Ser -26	-	expression tag
Gly -25	-	expression tag
Gly -24	-	expression tag
Gly -23	-	expression tag
Ser -22	-	expression tag
Gly -21	-	expression tag
Gly -20	-	expression tag
Ser -19	-	expression tag
Ala -18	-	expression tag
Trp -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
Pro -14	-	expression tag
Gln -13	-	expression tag
Phe -12	-	expression tag
Glu -11	-	expression tag
Lys -10	-	expression tag
Leu -9	-	expression tag
Glu -8	-	expression tag
Val -7	-	expression tag
Leu -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Pro -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Met 1	-	expression tag
Pro 2	-	expression tag
Lys 3	-	expression tag
Thr 4	-	expression tag
Gln 5	-	expression tag
Ala 6	-	expression tag
Glu 7	-	expression tag
Ile 8	-	expression tag
Leu 9	-	expression tag
Thr 10	-	expression tag
Leu 11	-	expression tag
Asp 12	-	expression tag
Phe 13	-	expression tag
Asn 14	-	expression tag
Leu 15	-	expression tag
Ala 16	-	expression tag
Glu 17	-	expression tag
Leu 18	-	expression tag
Pro 19	-	expression tag
Ser 20	-	expression tag
Ala 21	-	expression tag
Gln 22	-	expression tag
His 23	-	expression tag
Arg 24	-	expression tag
Ala 25	-	expression tag
Gly 26	-	expression tag
Leu 27	-	expression tag
Ala 28	-	expression tag
Gly 29	-	expression tag
Leu 30	-	expression tag
Ile 31	-	expression tag
Leu 32	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	15
	Total formula weight	404761.1
All*	Total formula weight	404761.1

*Water molecules are not included.