8H4D

Blasnase-T13A/M57N

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	L-asparaginase	polymer	329	36361.1	2	UniProt (A0A6I7U6Y2) Pfam (PF00710) Pfam (PF17763)	Bacillus paralicheniformis
2	C, D, E, I, J... (A, B)	MAGNESIUM ION	non-polymer		24.3	7	Chemie (MG)
3	F, G, M, N, O... (A, B)	FORMIC ACID	non-polymer		46.0	6	Chemie (FMT)
4	H (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5	Q, R (A, B)	water	water		18.0	483	Chemie (HOH)

Sequence modifications

A, B: 1 - 322 (UniProt: A0A6I7U6Y2)

PDB	External Database	Details
Ala 13	Thr 13	engineered mutation
Asn 57	Met 57	engineered mutation
Leu 323	-	expression tag
Glu 324	-	expression tag
His 325	-	expression tag
His 326	-	expression tag
His 327	-	expression tag
His 328	-	expression tag
His 329	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	72722.2
Non-Polymers*	Number of molecules	14
	Total formula weight	538.4
All*	Total formula weight	73260.6

*Water molecules are not included.