8H4D

Blasnase-T13A/M57N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	173
Detector technology	CCD
Collection date	2021-11-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	P 41 21 2
Unit cell lengths	91.643, 91.643, 232.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.020 - 1.900
Rwork	0.180
R-free	0.20690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7c8x
RMSD bond length	0.009
RMSD bond angle	0.927
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (v1.16)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.020	1.940
High resolution limit [Å]	1.900	1.900
Rpim	0.026	0.336
Number of reflections	78823	4403
<I/σ(I)>	17.3
Completeness [%]	100.0
Redundancy	25.1
CC(1/2)	0.999	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	sodium formate