8H45

Blasnase-T13A/P55N

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	L-asparaginase	polymer	329	36395.2	2	UniProt (A0A6I7U6Y2) Pfam (PF00710) Pfam (PF17763) In PDB	Bacillus paralicheniformis
2	A, B	MAGNESIUM ION	non-polymer		24.3	7	Chemie (MG)
3	A, B	FORMIC ACID	non-polymer		46.0	7	Chemie (FMT)
4		water	water		18.0	345	Chemie (HOH)

Sequence modifications

A, B: 1 - 322 (UniProt: A0A6I7U6Y2)

PDB	External Database	Details
Ala 13	Thr 13	engineered mutation
Asn 55	Pro 55	engineered mutation
Leu 323	-	expression tag
Glu 324	-	expression tag
His 325	-	expression tag
His 326	-	expression tag
His 327	-	expression tag
His 328	-	expression tag
His 329	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	72790.4
Non-Polymers*	Number of molecules	14
	Total formula weight	492.3
All*	Total formula weight	73282.7

*Water molecules are not included.