8H37
Cryo-EM Structure of the KBTBD2-CUL3-Rbx1-p85a tetrameric complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (C, F, M, O) | Cullin-3 | polymer | 768 | 89063.3 | 4 | UniProt (Q13618) Pfam (PF00888) Pfam (PF10557) | Homo sapiens (human) | CUL-3 |
| 2 | E, F, G, H (E, D, R, Q) | E3 ubiquitin-protein ligase RBX1 | polymer | 108 | 12290.0 | 4 | UniProt (P62877) Pfam (PF12678) | Homo sapiens (human) | E3 ubiquitin-protein transferase RBX1,Protein ZYP,RING finger protein 75,RING-box protein 1,Rbx1,Regulator of cullins 1,ROC1 |
| 3 | I, K, N, P (A, B, N, P) | Kelch repeat and BTB domain-containing protein 2 | polymer | 623 | 71431.4 | 4 | UniProt (Q8IY47) Pfam (PF00651) Pfam (PF07707) Pfam (PF01344) | Homo sapiens (human) | BTB and kelch domain-containing protein 1 |
| 4 | J, L, M, O (G, H, I, J) | Phosphatidylinositol 3-kinase regulatory subunit alpha | polymer | 724 | 83710.3 | 4 | UniProt (P27986) Pfam (PF00017) Pfam (PF00620) Pfam (PF16454) | Homo sapiens (human) | PI3-kinase regulatory subunit alpha,PI3K regulatory subunit alpha,PtdIns-3-kinase regulatory subunit alpha,Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha,PI3-kinase subunit p85-alpha,PtdIns-3-kinase regulatory subunit p85-alpha |
| 5 | AA, BA, Q, R, S... (Q, E, D, R) | ZINC ION | non-polymer | 65.4 | 12 | Chemie (ZN) |
Sequence modifications
A, B, N, P: 1 - 623 (UniProt: Q8IY47)
| PDB | External Database | Details |
|---|---|---|
| Asp 252 | Ser 252 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 16 |
| Total formula weight | 1025979.8 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 784.9 | |
| All* | Total formula weight | 1026764.8 |
