8H33

Cryo-EM Structure of the KBTBD2-Cul3-Rbx1 tetrameric complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, G, J)	Kelch repeat and BTB domain-containing protein 2	polymer	623	71403.4	4	UniProt (Q8IY47) Pfam (PF00651) Pfam (PF07707) Pfam (PF01344)	Homo sapiens (human)
2	E, H, J, L (D, K, L, E)	E3 ubiquitin-protein ligase RBX1	polymer	121	13970.8	4	UniProt (P62877) Pfam (PF12678)	Homo sapiens (human)	E3 ubiquitin-protein transferase RBX1,Protein ZYP,RING finger protein 75,RING-box protein 1,Rbx1,Regulator of cullins 1,ROC1
3	F, G, I, K (F, H, I, C)	Cullin-3	polymer	776	90105.5	4	UniProt (Q13618) Pfam (PF00888) Pfam (PF10557)	Homo sapiens (human)
4	M, N, O, P, Q... (D, K, L, E)	ZINC ION	non-polymer		65.4	12	Chemie (ZN)

Sequence modifications

D, K, L, E: 1 - 108 (UniProt: P62877)

PDB	External Database	Details
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag

F, H, I, C: 1 - 768 (UniProt: Q13618)

PDB	External Database	Details
Trp -7	-	expression tag
Ser -6	-	expression tag
His -5	-	expression tag
Pro -4	-	expression tag
Gln -3	-	expression tag
Phe -2	-	expression tag
Glu -1	-	expression tag
Lys 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	701918.4
Non-Polymers*	Number of molecules	12
	Total formula weight	784.9
All*	Total formula weight	702703.3

*Water molecules are not included.