8H2V

Cellodextrin phosphorylase from Clostridium thermocellum mutant - all cysteine residues were substituted with serines

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Cellodextrin phosphorylase	polymer	993	112736.2	2	UniProt (Q93HT8) Pfam (PF06165) Pfam (PF17167) In PDB	Acetivibrio thermocellus
2	A, B	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
3	A, B	beta-D-glucopyranose	non-polymer		180.2	2	Chemie (BGC)
4	B, A	GLYCEROL	non-polymer		92.1	15	Chemie (GOL)
5	B, A	ACETATE ION	non-polymer		59.0	11	Chemie (ACT)
6	B, A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	3	Chemie (PEG)
7	B, A	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
8		water	water		18.0	1557	Chemie (HOH)

Sequence modifications

A, B: 3 - 985 (UniProt: Q93HT8)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Ser 65	Cys 64	engineered mutation
Ser 81	Cys 80	engineered mutation
Ser 226	Cys 225	engineered mutation
Ser 230	Cys 229	engineered mutation
Ser 241	Cys 240	engineered mutation
Ser 354	Cys 353	engineered mutation
Ser 373	Cys 372	engineered mutation
Ser 607	Cys 606	engineered mutation
Ser 626	Cys 625	engineered mutation
Asp 630	Ala 629	engineered mutation
Ser 873	Cys 872	engineered mutation
Ser 935	Cys 934	engineered mutation
Leu 986	-	expression tag
Glu 987	-	expression tag
His 988	-	expression tag
His 989	-	expression tag
His 990	-	expression tag
His 991	-	expression tag
His 992	-	expression tag
His 993	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	225472.3
Non-Polymers*	Number of molecules	37
	Total formula weight	3041.3
All*	Total formula weight	228513.7

*Water molecules are not included.