8H2G

Cryo-EM structure of niacin bound human hydroxy-carboxylic acid receptor 2 in complex with Gi heterotrimer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Hydroxy-carboxylic acid receptor 2	polymer	476	54320.3	1	UniProt (Q8TDS4) Pfam (PF07361) Pfam (PF00001) UniProt (by SIFTS) (P0ABE7) In PDB	Homo sapiens (human)
2	B	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40415.0	1	UniProt (P63096) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
3	C	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	345	37728.2	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
4	D	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	80	8506.8	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
5	E	scFv16	polymer	257	27409.6	1		Mus musculus
6	A	NICOTINIC ACID	non-polymer		123.1	1	Chemie (NIO)

Sequence modifications

A: -112 - 0 (PDB: 8H2G)
A: 1 - 363 (UniProt: Q8TDS4)
C: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

D: 1 - 71 (UniProt: P59768)

PDB	External Database	Details
Gly 72	-	expression tag
Ser 73	-	expression tag
Ala 74	-	expression tag
Gly 75	-	expression tag
Ser 76	-	expression tag
Ala 77	-	expression tag
Gly 78	-	expression tag
Ser 79	-	expression tag
Ala 80	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	168379.8
Non-Polymers*	Number of molecules	1
	Total formula weight	123.1
All*	Total formula weight	168502.9

*Water molecules are not included.