8H1T

Cryo-EM structure of BAP1-ASXL1 bound to chromatosome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3.1	polymer	136	15437.2	2	UniProt (P68431) Pfam (PF00125)	Homo sapiens (human)
2	B, F (B, F)	Histone H4	polymer	103	11394.4	2	UniProt (P62805) Pfam (PF15511)	Homo sapiens (human)
3	C, G (C, G)	Histone H2A type 1-D	polymer	130	14137.5	2	UniProt (P20671) Pfam (PF00125) Pfam (PF16211)	Homo sapiens (human)	Histone H2A.3,Histone H2A/g
4	D, H (D, H)	Histone H2B type 2-E	polymer	126	13951.2	2	UniProt (Q16778) Pfam (PF00125)	Homo sapiens (human)	Histone H2B-GL105,Histone H2B.q,H2B/q
5	I (I)	DNA (187-MER)	polymer	187	57438.5	1		Homo sapiens (human)
6	J (J)	DNA (187-MER)	polymer	187	58031.0	1		Homo sapiens (human)
7	K (K)	Histone H1.4	polymer	229	23190.9	1	UniProt (P10412) Pfam (PF00538)	Homo sapiens (human)	Histone H1b,Histone H1s-4
8	L (L)	Ubiquitin carboxyl-terminal hydrolase BAP1	polymer	729	80456.6	1	UniProt (Q92560) Pfam (PF01088) Pfam (PF18031)	Homo sapiens (human)	BRCA1-associated protein 1,Cerebral protein 6
9	M (M)	Ubiquitin	polymer	76	8576.8	1	UniProt (P0CG47) Pfam (PF00240)	Homo sapiens (human)
10	N (N)	Polycomb group protein ASXL1	polymer	378	41788.6	1	UniProt (Q8IXJ9) Pfam (PF05066) Pfam (PF13919)	Homo sapiens (human)	Additional sex combs-like protein 1

Sequence modifications

K: 0 - 218 (UniProt: P10412)

PDB	External Database	Details
Met -2	-	initiating methionine
Gly -1	-	expression tag
Leu 219	-	expression tag
Glu 220	-	expression tag
His 221	-	expression tag
His 222	-	expression tag
His 223	-	expression tag
His 224	-	expression tag
His 225	-	expression tag
His 226	-	expression tag

L: 1 - 729 (UniProt: Q92560)

PDB	External Database	Details
Ser 91	Cys 91	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	379323.1
All*	Total formula weight	379323.1

*Water molecules are not included.