8GW8
the human PTH1 receptor bound to an intracellular biased agonist
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 359 | 42073.6 | 1 | UniProt (P63092) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-stimulating G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 351 | 38744.4 | 1 | UniProt (P54311) Pfam (PF25391) | Rattus norvegicus (Norway rat) | Transducin beta chain 1 |
| 3 | C (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 68 | 7547.7 | 1 | UniProt (P63212) Pfam (PF00631) | Bos taurus (cattle) | G gamma-I |
| 4 | D (N) | Nanobody-35 | polymer | 128 | 13885.4 | 1 | unidentified | ||
| 5 | E (R) | Parathyroid hormone/parathyroid hormone-related peptide receptor | polymer | 473 | 54264.4 | 1 | UniProt (Q03431) Pfam (PF02793) Pfam (PF00002) | Homo sapiens (human) | PTH/PTHrP type I receptor,PTH/PTHr receptor,Parathyroid hormone 1 receptor,PTH1 receptor |
| 6 | F (R) | PCO-371 | non-polymer | 635.7 | 1 | Chemie (KHF) |
Sequence modifications
A: 12 - 384 (UniProt: P63092)
B: 2 - 340 (UniProt: P54311)
G: 1 - 67 (UniProt: P63212)
R: 27 - 491 (UniProt: Q03431)
| PDB | External Database | Details |
|---|---|---|
| Asp 49 | Gly 49 | engineered mutation |
| Asn 50 | Glu 50 | engineered mutation |
| Asp 249 | Ala 235 | engineered mutation |
| Asp 252 | Ser 238 | engineered mutation |
| - | Asn 240 | deletion |
| - | Met 241 | deletion |
| - | Val 242 | deletion |
| - | Arg 244 | deletion |
| - | Glu 245 | deletion |
| - | Asp 246 | deletion |
| - | Asn 247 | deletion |
| - | Gln 248 | deletion |
| - | Thr 249 | deletion |
| Ala 362 | Ile 358 | engineered mutation |
| Ile 365 | Val 361 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser 68 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asp 19 | - | expression tag |
| Tyr 20 | - | expression tag |
| Lys 21 | - | expression tag |
| Asp 22 | - | expression tag |
| Asp 23 | - | expression tag |
| Asp 24 | - | expression tag |
| Asp 25 | - | expression tag |
| Lys 26 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 156515.4 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 635.7 | |
| All* | Total formula weight | 157151.1 |
