8GVX
Cryo-EM structure of the human TRPC5 ion channel in complex with G alpha i3 subunits, class2
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, F, G, H (B, A, C, D) | Short transient receptor potential channel 5 | polymer | 773 | 89951.9 | 4 | UniProt (Q9UL62) Pfam (PF12796) Pfam (PF00023) Pfam (PF08344) Pfam (PF00520) | Homo sapiens (human) | TrpC5,Transient receptor protein 5,TRP-5,hTRP-5,hTRP5 |
2 | B, C, D, E (F, E, G, H) | Guanine nucleotide-binding protein G(i) subunit alpha-3 | polymer | 360 | 41399.0 | 4 | UniProt (P08754) Pfam (PF00503) | Homo sapiens (human) | G(i) alpha-3 |
3 | BA, I, R, W (D, B, A, C) | PHOSPHATIDYLETHANOLAMINE | non-polymer | 734.0 | 4 | Chemie (PTY) | |||
4 | CA, J, S, X (D, B, A, C) | CHOLESTEROL HEMISUCCINATE | non-polymer | 486.7 | 4 | Chemie (Y01) | |||
5 | DA, K, T, Y (D, B, A, C) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) | |||
6 | EA, L, U, Z (D, B, A, C) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
7 | AA, FA, M, V (C, D, B, A) | (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate | non-polymer | 594.9 | 4 | Chemie (YZY) | |||
8 | N, O, P, Q (F, E, G, H) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 4 | Chemie (GTP) |
Sequence modifications
B, A, C, D: 1 - 765 (UniProt: Q9UL62)
F, E, G, H: 1 - 354 (UniProt: P08754)
PDB | External Database | Details |
---|---|---|
Ser 766 | - | expression tag |
Arg 767 | - | expression tag |
Leu 768 | - | expression tag |
Glu 769 | - | expression tag |
Val 770 | - | expression tag |
Leu 771 | - | expression tag |
Phe 772 | - | expression tag |
Gln 773 | - | expression tag |
PDB | External Database | Details |
---|---|---|
His 119 | - | insertion |
His 119 | - | insertion |
His 119 | - | insertion |
His 119 | - | insertion |
His 119 | - | insertion |
His 119 | - | insertion |
Leu 204 | Gln 204 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 8 |
Total formula weight | 525403.8 | |
Non-Polymers* | Number of molecules | 24 |
Total formula weight | 9777.5 | |
All* | Total formula weight | 535181.3 |