8GTI

Corticotropin-releasing hormone receptor 1(CRF1R) bound with BMK-C205 by XFEL

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Isoform CRF-R2 of Corticotropin-releasing factor receptor 1	polymer	284	32685.4	1	UniProt (P34998-2) Pfam (PF00002) UniProt (by SIFTS) (P34998) In PDB	Homo sapiens (human)	CRF-R-1,CRF-R1,CRFR-1,Corticotropin-releasing hormone receptor 1,CRH-R-1,CRH-R1
2	B	Endolysin	polymer	160	18113.7	1	UniProt (A0A346FJK3) Pfam (PF00959) In PDB	Enterobacteria phage T6	Lysis protein,Lysozyme,Muramidase
3	A	OLEIC ACID	non-polymer		282.5	5	Chemie (OLA)
4	A	8-(4-bromanyl-2,6-dimethoxy-phenyl)-~{N}-butyl-~{N}-(cyclopropylmethyl)-2,7-dimethyl-pyrazolo[1,5-a][1,3,5]triazin-4-amine	non-polymer		488.4	1	Chemie (0JS)

Sequence modifications

A: 104 - 373 (UniProt: P34998-2)

PDB	External Database	Details
Met 103	-	initiating methionine
Ala 120	Val 120	engineered mutation
Ala 144	Leu 144	engineered mutation
Ala 156	Trp 156	engineered mutation
Ala 160	Ser 160	engineered mutation
Ala 228	Lys 228	engineered mutation
Ala 260	Phe 260	engineered mutation
Ala 277	Ile 277	engineered mutation
Ala 309	Tyr 309	engineered mutation
Ala 330	Phe 330	engineered mutation
Ala 349	Ser 349	engineered mutation
Ala 363	Tyr 363	engineered mutation
Ala 374	-	expression tag
Ala 375	-	expression tag
Ala 376	-	expression tag
His 377	-	expression tag
His 378	-	expression tag
His 379	-	expression tag
His 380	-	expression tag
His 381	-	expression tag
His 382	-	expression tag
His 383	-	expression tag
His 384	-	expression tag
His 385	-	expression tag
His 386	-	expression tag

B: 1000 - 1159 (UniProt: A0A346FJK3)

PDB	External Database	Details
Ser 1052	Cys 54	engineered mutation
Ser 1095	Cys 97	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	50799.1
Non-Polymers*	Number of molecules	6
	Total formula weight	1900.7
All*	Total formula weight	52699.8

*Water molecules are not included.