8GQE
Crystal structure of the W285A mutant of UVR8 in complex with RUP2
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | WD repeat-containing protein RUP2 | polymer | 369 | 40236.7 | 1 | UniProt (Q9FFA7) Pfam (PF00400) | Arabidopsis thaliana (thale cress) | Protein EARLY FLOWERING BY OVEREXPRESSION 2,Protein REPRESSOR OF UV-B PHOTOMORPHOGENESIS 2 |
| 2 | B (B) | Ultraviolet-B receptor UVR8 | polymer | 443 | 47269.2 | 1 | UniProt (Q9FN03) Pfam (PF00415) Pfam (PF25390) | Arabidopsis thaliana (thale cress) | Protein UV-B RESISTANCE 8,RCC1 domain-containing protein UVR8 |
| 3 | C (B) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 1 | Chemie (MES) | |||
| 4 | D, E (A, B) | water | water | 18.0 | 450 | Chemie (HOH) |
Sequence modifications
A: 20 - 366 (UniProt: Q9FFA7)
B: 12 - 421 (UniProt: Q9FN03)
| PDB | External Database | Details |
|---|---|---|
| Met 19 | - | initiating methionine |
| Leu 367 | - | expression tag |
| Glu 368 | - | expression tag |
| Asp 369 | - | expression tag |
| Tyr 370 | - | expression tag |
| Lys 371 | - | expression tag |
| Asp 372 | - | expression tag |
| His 373 | - | expression tag |
| Asp 374 | - | expression tag |
| Gly 375 | - | expression tag |
| Asp 376 | - | expression tag |
| Tyr 377 | - | expression tag |
| Lys 378 | - | expression tag |
| Asp 379 | - | expression tag |
| His 380 | - | expression tag |
| Asp 381 | - | expression tag |
| Ile 382 | - | expression tag |
| Asp 383 | - | expression tag |
| Tyr 384 | - | expression tag |
| Lys 385 | - | expression tag |
| Asp 386 | - | expression tag |
| Asp 387 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 11 | - | initiating methionine |
| Ala 285 | Trp 285 | engineered mutation |
| Leu 422 | - | expression tag |
| Glu 423 | - | expression tag |
| Ser 424 | - | expression tag |
| Ala 425 | - | expression tag |
| Trp 426 | - | expression tag |
| Ser 427 | - | expression tag |
| His 428 | - | expression tag |
| Pro 429 | - | expression tag |
| Gln 430 | - | expression tag |
| Phe 431 | - | expression tag |
| Glu 432 | - | expression tag |
| Lys 433 | - | expression tag |
| Gly 434 | - | expression tag |
| Gly 435 | - | expression tag |
| Gly 436 | - | expression tag |
| Ser 437 | - | expression tag |
| Gly 438 | - | expression tag |
| Gly 439 | - | expression tag |
| Gly 440 | - | expression tag |
| Ser 441 | - | expression tag |
| Gly 442 | - | expression tag |
| Gly 443 | - | expression tag |
| Ser 444 | - | expression tag |
| Ala 445 | - | expression tag |
| Trp 446 | - | expression tag |
| Ser 447 | - | expression tag |
| His 448 | - | expression tag |
| Pro 449 | - | expression tag |
| Gln 450 | - | expression tag |
| Phe 451 | - | expression tag |
| Glu 452 | - | expression tag |
| Lys 453 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 87505.9 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 195.2 | |
| All* | Total formula weight | 87701.1 |
