8GQE

Crystal structure of the W285A mutant of UVR8 in complex with RUP2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	I 4
Unit cell lengths	173.393, 173.393, 76.737
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.010 - 2.000
R-factor	0.1747
Rwork	0.173
R-free	0.20030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dnv
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.070
High resolution limit [Å]	2.000	2.000
Rmeas	0.166	0.884
Rpim	0.044	0.241
Number of reflections	76777	7644
<I/σ(I)>	23.9
Completeness [%]	100.0
Redundancy	13.8
CC(1/2)	0.957	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291.15	PEG 6000, Ammonium chloride, Hexamminecobalt (III) chloride, sucrose