8GOG
Structure of streptavidin mutant (S112Y-K121E) complexed with biotin-cyclopentadienyl-rhodium (III)(Cp*-Rh(III))
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Streptavidin | polymer | 121 | 12857.8 | 2 | UniProt (P22629) Pfam (PF01382) | Streptomyces avidinii | |
| 2 | C, E, N, O (A, B) | RHODIUM(III) ION | non-polymer | 102.9 | 4 | Chemie (RH3) | |||
| 3 | D, P (A, B) | trichloro((3~{a}~{S},4~{S},6~{a}~{R})-4-[(5~{R})-5-oxidanyl-5-[2-(2,3,4,5-tetramethylcyclopenta-2,4-dien-1-ylidene)ethylamino]pentyl]-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-2-one)rhodium(3+) | non-polymer | 599.8 | 2 | Chemie (JSU) | |||
| 4 | F, J, K, R, S... (A, B) | GLYCEROL | non-polymer | 92.1 | 9 | Chemie (GOL) | |||
| 5 | G, H, I, Q (A, B) | CHLORIDE ION | non-polymer | 35.5 | 4 | Chemie (CL) | |||
| 6 | L, M, X, Y, Z (A, B) | SULFATE ION | non-polymer | 96.1 | 5 | Chemie (SO4) | |||
| 7 | AA, BA (A, B) | water | water | 18.0 | 66 | Chemie (HOH) |
Sequence modifications
A, B: 15 - 133 (UniProt: P22629)
| PDB | External Database | Details |
|---|---|---|
| Asp 13 | - | expression tag |
| Gln 14 | - | expression tag |
| Tyr 112 | Ser 136 | engineered mutation |
| Glu 121 | Lys 145 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 25715.6 | |
| Non-Polymers* | Number of molecules | 24 |
| Total formula weight | 3062.3 | |
| All* | Total formula weight | 28777.8 |
