8GOG
Structure of streptavidin mutant (S112Y-K121E) complexed with biotin-cyclopentadienyl-rhodium (III)(Cp*-Rh(III))
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2022-01-12 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 79.450, 81.390, 90.250 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.730 - 2.000 |
R-factor | 0.2532 |
Rwork | 0.251 |
R-free | 0.29420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5cse |
RMSD bond length | 0.010 |
RMSD bond angle | 1.312 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.310 | |
Number of reflections | 19868 | 2711 |
<I/σ(I)> | 4 | |
Completeness [%] | 100.0 | |
Redundancy | 7 | |
CC(1/2) | 0.990 | 0.530 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.7 | 291 | 0.2 M sodium sulfate pH 6.7 with 20% w/v PEG 3350 |