8GKH

Structure of the Spizellomyces punctatus Fanzor (SpuFz) in complex with omega RNA and target DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (N)	DNA (5'-D(P*CP*GP*GP*TP*AP*CP*CP*CP*GP*GP*GP*CP*AP*TP*A)-3')	polymer	15	4595.0	1		synthetic construct
2	B (P)	Maltodextrin-binding protein (Fragment),OrfB_Zn_ribbon domain-containing protein	polymer	1032	118029.9	1	UniProt (A0A0F8NYV9) UniProt (A0A0L0H5U9) Pfam (PF01547) UniProt (by SIFTS) (P0AEX9)	Methanosarcina mazei
3	C (T)	DNA (35-MER)	polymer	35	10737.9	1		synthetic construct
4	D (W)	RNA (78-MER)	polymer	78	25050.8	1		Spizellomyces punctatus
5	E, F (P)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	G (P)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
7	H, I, J (P, T, W)	water	water		18.0	3	Chemie (HOH)

Sequence modifications

P: -376 - -8 (UniProt: A0A0F8NYV9)

PDB	External Database	Details
Met -393	-	initiating methionine
Lys -392	-	expression tag
Ser -391	-	expression tag
Ser -390	-	expression tag
His -389	-	expression tag
His -388	-	expression tag
His -387	-	expression tag
His -386	-	expression tag
His -385	-	expression tag
His -384	-	expression tag
His -383	-	expression tag
His -382	-	expression tag
His -381	-	expression tag
His -380	-	expression tag
Gly -379	-	expression tag
Ser -378	-	expression tag
Ser -377	-	expression tag
Ile -374	Thr 3	conflict
Gly -9	Asn 368	conflict
Glu -7	-	linker
Asn -6	-	linker
Leu -5	-	linker
Tyr -4	-	linker
Phe -3	-	linker
Gln -2	-	linker
Ser -1	-	linker
Asn 0	-	linker
Ala 1	-	linker

P: 2 - 638 (UniProt: A0A0L0H5U9)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	158413.6
Non-Polymers*	Number of molecules	3
	Total formula weight	114.0
All*	Total formula weight	158527.7

*Water molecules are not included.