8GDU

Crystal structure of a mutant methyl transferase from Methanosarcina acetivorans, Northeast Structural Genomics Consortium (NESG) Target MvR53-11M

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Methyltransferase domain-containing protein	polymer	202	23371.5	1	UniProt (Q8TNZ0) Pfam (PF13649)	Methanosarcina acetivorans C2A
2	B (A)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	1	Chemie (SAH)
3	C (A)	water	water		18.0	89	Chemie (HOH)

Sequence modifications

A: 1 - 194 (UniProt: Q8TNZ0)

PDB	External Database	Details
Arg 8	Lys 8	engineered mutation
Arg 52	Lys 52	engineered mutation
Arg 64	Lys 64	engineered mutation
Arg 65	Asp 65	engineered mutation
Arg 71	Lys 71	engineered mutation
Arg 126	Lys 126	engineered mutation
Arg 129	Lys 129	engineered mutation
Arg 133	Lys 133	engineered mutation
Arg 142	Lys 142	engineered mutation
Arg 155	Lys 155	engineered mutation
Arg 172	Lys 172	engineered mutation
Arg 194	Lys 194	engineered mutation
Leu 195	-	expression tag
Glu 196	-	expression tag
His 197	-	expression tag
His 198	-	expression tag
His 199	-	expression tag
His 200	-	expression tag
His 201	-	expression tag
His 202	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	23371.5
Non-Polymers*	Number of molecules	1
	Total formula weight	384.4
All*	Total formula weight	23755.9

*Water molecules are not included.