8GDU
Crystal structure of a mutant methyl transferase from Methanosarcina acetivorans, Northeast Structural Genomics Consortium (NESG) Target MvR53-11M
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-27 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 109.950, 109.950, 39.124 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 77.750 - 2.010 |
| R-factor | 0.185 |
| Rwork | 0.182 |
| R-free | 0.21030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.035 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.17) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 109.950 | 1.950 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.135 | 2.828 |
| Number of reflections | 19350 | 1253 |
| <I/σ(I)> | 20.4 | |
| Completeness [%] | 99.8 | 97.7 |
| Redundancy | 25.4 | |
| CC(1/2) | 0.999 | 0.491 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 277 | 0.1 M HEPES and 30% (w/v) PEG 1k |
