8GCB

Structure of RNF125 in complex with a UbcH5b~Ub conjugate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ubiquitin-conjugating enzyme E2 D2	polymer	152	17214.6	1	UniProt (P62837) Pfam (PF00179)	Homo sapiens (human)	(E3-independent) E2 ubiquitin-conjugating enzyme D2,E2 ubiquitin-conjugating enzyme D2,Ubiquitin carrier protein D2,Ubiquitin-conjugating enzyme E2(17)KB 2,Ubiquitin-conjugating enzyme E2-17 kDa 2,Ubiquitin-protein ligase D2,p53-regulated ubiquitin-conjugating enzyme 1
2	B (B)	E3 ubiquitin-protein ligase RNF125	polymer	101	11345.2	1	UniProt (Q96EQ8) Pfam (PF13923) Pfam (PF18574)	Homo sapiens (human)	RING finger protein 125,T-cell RING activation protein 1,TRAC-1
3	C, D, E (B)	ZINC ION	non-polymer		65.4	3	Chemie (ZN)

Sequence modifications

A: 1 - 147 (UniProt: P62837)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ser 21	Cys 21	engineered mutation
Arg 22	Ser 22	engineered mutation
Lys 85	Cys 85	engineered mutation
Ser 107	Cys 107	engineered mutation
Ser 111	Cys 111	engineered mutation

B: 32 - 127 (UniProt: Q96EQ8)

PDB	External Database	Details
Gly 27	-	expression tag
Pro 28	-	expression tag
Leu 29	-	expression tag
Gly 30	-	expression tag
Ser 31	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	28559.9
Non-Polymers*	Number of molecules	3
	Total formula weight	196.2
All*	Total formula weight	28756.1

*Water molecules are not included.