8GCB

Structure of RNF125 in complex with a UbcH5b~Ub conjugate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953646
Spacegroup name	P 43 21 2
Unit cell lengths	59.325, 59.325, 185.821
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.500 - 2.390
R-factor	0.2536
Rwork	0.253
R-free	0.27080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.361
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.500	46.460	2.480
High resolution limit [Å]	2.390	8.940	2.390
Rmerge	0.120	0.050	2.507
Rmeas	0.123	0.051	2.560
Rpim	0.024	0.011	0.506
Total number of observations		6871	35075
Number of reflections	13972	344	1418
<I/σ(I)>	16.8	49.6	1.5
Completeness [%]	99.8	99.5	98.6
Redundancy	25.1	20	24.7
CC(1/2)	0.999	0.999	0.684

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	9	289	0.1 M BICINE pH 9.0, 20% PEG 6000