8GCB
Structure of RNF125 in complex with a UbcH5b~Ub conjugate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.953646 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 59.325, 59.325, 185.821 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.500 - 2.390 |
| R-factor | 0.2536 |
| Rwork | 0.253 |
| R-free | 0.27080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.361 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.500 | 46.460 | 2.480 |
| High resolution limit [Å] | 2.390 | 8.940 | 2.390 |
| Rmerge | 0.120 | 0.050 | 2.507 |
| Rmeas | 0.123 | 0.051 | 2.560 |
| Rpim | 0.024 | 0.011 | 0.506 |
| Total number of observations | 6871 | 35075 | |
| Number of reflections | 13972 | 344 | 1418 |
| <I/σ(I)> | 16.8 | 49.6 | 1.5 |
| Completeness [%] | 99.8 | 99.5 | 98.6 |
| Redundancy | 25.1 | 20 | 24.7 |
| CC(1/2) | 0.999 | 0.999 | 0.684 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 9 | 289 | 0.1 M BICINE pH 9.0, 20% PEG 6000 |
