8GB5

Crystal structure of SARS-CoV-2 receptor binding domain in complex with neutralizing antibody 25F9

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, G, J (A, D, G, J)	Spike protein S1	polymer	205	23104.9	4	UniProt (P0DTC2) Pfam (PF09408)	Severe acute respiratory syndrome coronavirus 2
2	B, F, I, L (B, F, I, L)	25F9 Heavy chain	polymer	231	24788.9	4		Macaca mulatta (Rhesus monkey)
3	C, E, H, K (C, E, H, K)	25F9 Light chain	polymer	216	22774.1	4		Macaca mulatta (Rhesus monkey)
4	M (M)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
5	N (N)	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	1	In PDB GlyTouCan (G22768VO)
6	O, P (O, P)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		732.7	2	In PDB GlyTouCan (G32152BH)
7	AA, BA, Q, S, T... (K, A, D, E, I...)	SULFATE ION	non-polymer		96.1	8	Chemie (SO4)
8	R, V, W (C, E, H)	BICINE	non-polymer		163.2	3	Chemie (BCN)
9	Z (K)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
10	CA, DA, EA, FA, GA... (A, B, C, D, E...)	water	water		18.0	61	Chemie (HOH)

Sequence modifications

A, D, G, J: 333 - 530 (UniProt: P0DTC2)

PDB	External Database	Details
Gly 531	-	expression tag
His 532	-	expression tag
His 533	-	expression tag
His 534	-	expression tag
His 535	-	expression tag
His 536	-	expression tag
His 537	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	282671.6
Branched	Number of molecules	4
	Total formula weight	2962.7
Non-Polymers*	Number of molecules	12
	Total formula weight	1350.1
All*	Total formula weight	286984.4

*Water molecules are not included.