8GAM
Exploiting Activation and Inactivation Mechanisms in Type I-C CRISPR-Cas3 for Genome Editing Applications
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... | Cas7 | polymer | 283 | 32208.1 | 7 | UniProt (A0A378VEU0) Pfam (PF05107) UniProt (by SIFTS) (D0W8X6) In PDB | Neisseria lactamica | |
| 2 | G, H | Phage associated protein | polymer | 582 | 64715.7 | 2 | UniProt (A0A378VF47) Pfam (PF09709) UniProt (by SIFTS) (D0W8X5) In PDB | Neisseria lactamica | |
| 3 | I, J | Cas11 | polymer | 124 | 14245.2 | 2 | UniProt (A0A378VF47) UniProt (by SIFTS) (D0W8X5) In PDB | Neisseria lactamica | |
| 4 | K | crRNA (43-MER) | polymer | 43 | 13816.2 | 1 | Neisseria lactamica | ||
| 5 | N | Cas5 | polymer | 205 | 23870.5 | 1 | UniProt (D0W8X4) Pfam (PF09704) In PDB | Neisseria lactamica | |
| 6 | L | Target strand DNA (25-MER) | polymer | 25 | 7658.9 | 1 | Neisseria lactamica | ||
| 7 | O | Non target strand DNA (5'-D(P*AP*TP*GP*AP*AP*CP*TP*TP*CP*AP*AP*AP*A)-3') | polymer | 13 | 3967.6 | 1 | Neisseria lactamica |
Sequence modifications
A, B, C, D, E, F, M: 2 - 283 (UniProt: A0A378VEU0)
G, H: 1 - 582 (UniProt: A0A378VF47)
| PDB | External Database | Details |
|---|---|---|
| Gly 284 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ala 190 | Val 190 | conflict |
| Ala 239 | Ile 239 | conflict |
| Ile 242 | Val 242 | conflict |
| Gly 260 | Ser 260 | conflict |
| Thr 271 | Ala 271 | conflict |
| Leu 288 | Met 288 | conflict |
| Ala 299 | Glu 299 | conflict |
| Ala 306 | Thr 306 | conflict |
| Cys 317 | Gln 317 | conflict |
| Glu 322 | Lys 322 | conflict |
| Asp 323 | Glu 323 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 15 |
| Total formula weight | 432691.7 | |
| All* | Total formula weight | 432691.7 |
