8G9Z

High-resolution crystal structure of the human selenomethionine-derived SepSecS-tRNASec complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	O-phosphoseryl-tRNA(Sec) selenium transferase	polymer	521	58741.7	4	UniProt (Q9HD40) Pfam (PF05889)	Homo sapiens (human)	Liver-pancreas antigen,LP,SLA-p35,SLA/LP autoantigen,Selenocysteine synthase,Sec synthase,Selenocysteinyl-tRNA(Sec) synthase,Sep-tRNA:Sec-tRNA synthase,SepSecS,Soluble liver antigen,SLA,UGA suppressor tRNA-associated protein,tRNA(Ser/Sec)-associated antigenic protein
2	E (E)	RNA (90-MER)	polymer	90	28908.1	1	GenBank (436409)	Homo sapiens
3	F, H, J, L (A, B, C, D)	(5-HYDROXY-4,6-DIMETHYLPYRIDIN-3-YL)METHYL DIHYDROGEN PHOSPHATE	non-polymer		233.2	4	Chemie (PLR)
4	G, I, K, M (A, B, C, D)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	4	Chemie (MPD)
5	N, O, P, Q, R (A, B, C, D, E)	water	water		18.0	1331	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 501 (UniProt: Q9HD40)

PDB	External Database	Details
Mse -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 491	Val 491	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	263875.0
Non-Polymers*	Number of molecules	8
	Total formula weight	1405.3
All*	Total formula weight	265280.3

*Water molecules are not included.