8G9S

Exploiting Activation and Inactivation Mechanisms in Type I-C CRISPR-Cas3 for Genome Editing Applications

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (B, C, D, E, F...)	Cas7	polymer	283	32208.1	7	UniProt (A0A378VEU0) Pfam (PF05107) UniProt (by SIFTS) (D0W8X6)	Neisseria lactamica
2	G, H, I, N (H, I, J, L)	Cas11	polymer	124	14245.2	4	UniProt (A0A378VF47) UniProt (by SIFTS) (D0W8X5)	Neisseria lactamica
3	K (N)	Cas5	polymer	205	23854.5	1	UniProt (D0W8X4) Pfam (PF09704)	Neisseria lactamica
4	L (O)	RNA (42-MER)	polymer	42	13495.0	1		Neisseria lactamica
5	M (K)	Cas8	polymer	409	45864.5	1	UniProt (A0A378VF47) Pfam (PF09709) UniProt (by SIFTS) (D0W8X5)	Neisseria lactamica
6	O (A)	AcrIC8	polymer	70	8190.2	1		Rhodobacter phage RcNL1

Sequence modifications

B, C, D, E, F, G, M: 2 - 283 (UniProt: A0A378VEU0)

PDB	External Database	Details
Gly 284	-	expression tag

N: 2 - 206 (UniProt: D0W8X4)

PDB	External Database	Details
Ala 32	Ser 32	conflict

K: 6 - 582 (UniProt: A0A378VF47)

PDB	External Database	Details
Met 1	-	initiating methionine
Ile 2	-	expression tag
Leu 3	-	expression tag
His 4	-	expression tag
Ala 5	-	expression tag
Leu 6	Val 179	conflict
Thr 7	Ser 180	conflict
Tyr 9	Ala 182	conflict
Tyr 10	Asn 183	conflict
Arg 12	Thr 185	conflict
Lys 13	Gln 186	conflict
Ala 14	Ser 187	conflict
Glu 15	Asp 188	conflict
Ser 16	Asn 189	conflict
Ala 190	Val 190	conflict
Ala 239	Ile 239	conflict
Ile 242	Val 242	conflict
Gly 260	Ser 260	conflict
Thr 271	Ala 271	conflict

Contents of the asymmetric unit

Polymers	Number of chains	15
	Total formula weight	373841.7
All*	Total formula weight	373841.7

*Water molecules are not included.