8G94
Structure of CD69-bound S1PR1 coupled to heterotrimeric Gi
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Sphingosine 1-phosphate receptor 1 | polymer | 355 | 40083.8 | 1 | UniProt (P21453) Pfam (PF00001) | Homo sapiens (human) | S1P receptor 1,S1P1,Endothelial differentiation G-protein coupled receptor 1,Sphingosine 1-phosphate receptor Edg-1,S1P receptor Edg-1 |
| 2 | B (B) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40445.1 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 3 | C (C) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37728.2 | 1 | UniProt (P62873) Pfam (PF25391) | Homo sapiens (human) | Transducin beta chain 1 |
| 4 | D (D) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 5 | E (E) | scFv16 | polymer | 259 | 27784.9 | 1 | Mus musculus | ||
| 6 | F, G (F, G) | Early activation antigen CD69 | polymer | 211 | 23912.8 | 2 | UniProt (Q07108) Pfam (PF00059) | Homo sapiens (human) | Activation inducer molecule,AIM,BL-AC/P26,C-type lectin domain family 2 member C,EA1,Early T-cell activation antigen p60,GP32/28,Leukocyte surface antigen Leu-23,MLR-3 |
Sequence modifications
A: 1 - 347 (UniProt: P21453)
B: 1 - 354 (UniProt: P63096)
C: 2 - 340 (UniProt: P62873)
F, G: 3 - 199 (UniProt: Q07108)
| PDB | External Database | Details |
|---|---|---|
| Asp 348 | - | expression tag |
| Tyr 349 | - | expression tag |
| Lys 350 | - | expression tag |
| Asp 351 | - | expression tag |
| Asp 352 | - | expression tag |
| Asp 353 | - | expression tag |
| Asp 354 | - | expression tag |
| Lys 355 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ala 203 | Gly 203 | conflict |
| Ser 326 | Ala 326 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | expression tag |
| Ala 2 | - | expression tag |
| Gly 200 | - | expression tag |
| Ser 201 | - | expression tag |
| Ala 202 | - | expression tag |
| Ser 203 | - | expression tag |
| Trp 204 | - | expression tag |
| Ser 205 | - | expression tag |
| His 206 | - | expression tag |
| Pro 207 | - | expression tag |
| Gln 208 | - | expression tag |
| Phe 209 | - | expression tag |
| Glu 210 | - | expression tag |
| Lys 211 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 7 |
| Total formula weight | 201728.6 | |
| All* | Total formula weight | 201728.6 |
