8G8B

Nucleosome with human nMatn1 sequence in complex with Human Oct4

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E	Histone H3	polymer	135	15303.9	2	UniProt (P84233) Pfam (PF00125) In PDB	Xenopus laevis (African clawed frog)
2	B, F	Histone H4	polymer	102	11263.2	2	UniProt (P62799) Pfam (PF15511) In PDB	Xenopus laevis (African clawed frog)
3	C, G	Histone H2A	polymer	129	13978.2	2	UniProt (Q6AZJ8) Pfam (PF00125) Pfam (PF16211) In PDB	Xenopus laevis (African clawed frog)
4	D, H	Histone H2B	polymer	122	13524.8	2	UniProt (P02281) Pfam (PF00125) In PDB	Xenopus laevis (African clawed frog)	H2B1.1
5	I	nMatn1 DNA (top strand,168-MER)	polymer	186	56933.6	1		Homo sapiens
6	J	nMatn1 DNA (bottom strand, 168-MER)	polymer	186	57889.9	1		Homo sapiens
7	X	POU domain, class 5, transcription factor 1	polymer	395	42299.5	1	UniProt (Q01860) Pfam (PF00157) Pfam (PF00046) In PDB	Homo sapiens (human)	Octamer-binding protein 3,Oct-3,Octamer-binding protein 4,Oct-4,Octamer-binding transcription factor 3,OTF-3

Sequence modifications

A, E: 1 - 135 (UniProt: P84233)

PDB	External Database	Details
Ala 102	Gly 103	variant

D, H: 1 - 122 (UniProt: P02281)

PDB	External Database	Details
Thr 29	Ser 33	variant

X: 1 - 360 (UniProt: Q01860)

PDB	External Database	Details
Gly -34	-	expression tag
Ser -33	-	expression tag
Ser -32	-	expression tag
His -31	-	expression tag
His -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
Ser -25	-	expression tag
Ser -24	-	expression tag
Gly -23	-	expression tag
Leu -22	-	expression tag
Val -21	-	expression tag
Pro -20	-	expression tag
Arg -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
Met -15	-	expression tag
Ala -14	-	expression tag
Ser -13	-	expression tag
Met -12	-	expression tag
Thr -11	-	expression tag
Gly -10	-	expression tag
Gly -9	-	expression tag
Gln -8	-	expression tag
Gln -7	-	expression tag
Met -6	-	expression tag
Gly -5	-	expression tag
Arg -4	-	expression tag
Asp -3	-	expression tag
Pro -2	-	expression tag
Asn -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	265263.2
All*	Total formula weight	265263.2

*Water molecules are not included.