8G7Z

Cryo-EM structure of full length Neuroligin-2 from Mouse bound to two Neurexin-1 Beta conformation one

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Neuroligin-2	polymer	870	95226.8	2	UniProt (Q62888) Pfam (PF00135) UniProt (by SIFTS) (Q69ZK9)	Mus musculus (house mouse)
2	C, D (C, D)	Neurexin-1	polymer	470	50435.3	2	UniProt (E0CZA5) Pfam (PF02210) Pfam (PF01034)	Mus musculus (house mouse)
3	E, F, G, H, J... (A, B, C, D)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)
4	I, K (C, D)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)

Sequence modifications

A, B: 14 - 836 (UniProt: Q62888)

PDB	External Database	Details
Met -41	-	initiating methionine
Ala -40	-	expression tag
Leu -39	-	expression tag
Pro -38	-	expression tag
Arg -37	-	expression tag
Cys -36	-	expression tag
Met -35	-	expression tag
Trp -34	-	expression tag
Pro -33	-	expression tag
Asn -32	-	expression tag
Tyr -31	-	expression tag
Val -30	-	expression tag
Trp -29	-	expression tag
Arg -28	-	expression tag
Ala -27	-	expression tag
Met -26	-	expression tag
Met -25	-	expression tag
Ala -24	-	expression tag
Cys -23	-	expression tag
Val -22	-	expression tag
Val -21	-	expression tag
His -20	-	expression tag
Arg -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Gly -16	-	expression tag
Ala -15	-	expression tag
Pro -14	-	expression tag
Leu -13	-	expression tag
Thr -12	-	expression tag
Leu -11	-	expression tag
Cys -10	-	expression tag
Leu -9	-	expression tag
Leu -8	-	expression tag
Gly -7	-	expression tag
Cys -6	-	expression tag
Leu -5	-	expression tag
Leu -4	-	expression tag
Gln -3	-	expression tag
Thr -2	-	expression tag
Phe -1	-	expression tag
His 0	-	expression tag
Val 1	-	expression tag
Leu 2	-	expression tag
Ser 3	-	expression tag
Gln 4	-	expression tag
Lys 5	-	expression tag
Tyr 6	-	expression tag
Pro 7	-	expression tag
Tyr 8	-	expression tag
Asp 9	-	expression tag
Val 10	-	expression tag
Pro 11	-	expression tag
Asp 12	-	expression tag
Tyr 13	-	expression tag
Val 210	Ala 193	conflict
Ser 837	-	expression tag
Asn 838	-	expression tag
Ser 839	-	expression tag
Leu 840	-	expression tag
Glu 841	-	expression tag
Val 842	-	expression tag
Leu 843	-	expression tag
Phe 844	-	expression tag
Gln 845	-	expression tag

C, D: -22 - 39 (UniProt: E0CZA5)

PDB	External Database	Details
Gly 15	Ser 38	conflict

C, D: 68 - 468 (UniProt: E0CZA5)

PDB	External Database	Details
Lys 40	-	insertion
Glu 41	-	insertion
Phe 42	-	insertion
Glu 43	-	insertion
Gln 44	-	insertion
Lys 45	-	insertion
Leu 46	-	insertion
Ile 47	-	insertion
Ser 48	-	insertion
Glu 49	-	insertion
Glu 50	-	insertion
Asp 51	-	insertion
Leu 52	-	insertion
Gly 53	-	insertion
Phe 54	-	insertion
Glu 55	-	insertion
Ile 56	-	insertion
Asp 57	-	insertion
Lys 58	-	insertion
Val 59	-	insertion
Trp 60	-	insertion
His 61	-	insertion
Asp 62	-	insertion
Phe 63	-	insertion
Pro 64	-	insertion
Ala 65	-	insertion
Thr 66	-	insertion
Ser 67	-	insertion
Ser 469	-	expression tag
Asn 470	-	expression tag
Ser 471	-	expression tag
Leu 472	-	expression tag
Glu 473	-	expression tag
Val 474	-	expression tag
Leu 475	-	expression tag
Phe 476	-	expression tag
Gln 477	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	291324.3
Non-Polymers*	Number of molecules	8
	Total formula weight	1407.4
All*	Total formula weight	292731.7

*Water molecules are not included.