8G6U
Cryo-EM structure of T/F100 SOSIP.664 HIV-1 Env trimer with LMHS mutations in complex with 8ANC195 and 10-1074
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, G, M (A, E, I) | CRF01_AE T/F100 HIV-1 gp120 | polymer | 485 | 54697.1 | 3 | UniProt (A0A6C0ZY47) | Human immunodeficiency virus 1 | |
| 2 | B, H, N (B, F, J) | CRF-1_AE T/F100 HIV-1 gp41 | polymer | 155 | 17389.8 | 3 | UniProt (A0A6C0ZY47) UniProt (by SIFTS) (B0EZY8) | Human immunodeficiency virus 1 | Env polyprotein |
| 3 | C, I, O (C, G, K) | Heavy chain of 8ANC195 | polymer | 238 | 25324.4 | 3 | Homo sapiens (human) | ||
| 4 | D, J, P (D, H, L) | Light chain of 8ANC195 | polymer | 215 | 23460.0 | 3 | Homo sapiens (human) | ||
| 5 | E, K, Q (M, O, Q) | Heavy chain of 10-1074 | polymer | 238 | 25661.7 | 3 | Homo sapiens (human) | ||
| 6 | F, L, R (N, P, R) | Light chain of 10-1074 | polymer | 214 | 23180.8 | 3 | Homo sapiens (human) | ||
| 7 | IA, S, Z (j, S, a) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1883.7 | 3 | In PDB GlyTouCan (G60230HH) | |||
| 8 | AA, GA, JA, QA, T... (b, h, k, r, T...) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 6 | In PDB GlyTouCan (G56014GC) | |||
| 9 | CA, MA, U (d, n, U) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1721.5 | 3 | In PDB GlyTouCan (G40702WU) | |||
| 10 | AB, BA, BB, DA, EA... (1, c, 2, f, e...) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 21 | In PDB GlyTouCan (G42666HT) | |||
| 11 | HA, RA, Y (i, s, Z) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 3 | In PDB GlyTouCan (G22768VO) | |||
| 12 | AC, BC, CB, CC, DB... (I, A, J, B, E...) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 36 | Chemie (NAG) |
Sequence modifications
A, E, I: 30 - 507 (UniProt: A0A6C0ZY47)
B, F, J: 512 - 664 (UniProt: A0A6C0ZY47)
| PDB | External Database | Details |
|---|---|---|
| Tyr 61 | His 60 | engineered mutation |
| His 105 | Gln 104 | engineered mutation |
| Ile 108 | Val 107 | engineered mutation |
| Ser 375 | His 381 | conflict |
| - | Ser 462 | deletion |
| Asp 474 | Asn 475 | engineered mutation |
| Met 475 | Ile 476 | engineered mutation |
| Arg 476 | Lys 477 | engineered mutation |
| Cys 501 | Ala 502 | conflict |
| Arg 508 | - | expression tag |
| Arg 509 | - | expression tag |
| Arg 510 | - | expression tag |
| Arg 511 | - | expression tag |
| Arg 512 | - | expression tag |
| Arg 513 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Pro 559 | Ile 560 | conflict |
| Cys 605 | Thr 606 | conflict |
| Ala 665 | - | expression tag |
| Ala 666 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 18 |
| Total formula weight | 509141.3 | |
| Branched | Number of molecules | 36 |
| Total formula weight | 28898.3 | |
| Non-Polymers* | Number of molecules | 36 |
| Total formula weight | 7963.5 | |
| All* | Total formula weight | 546003.1 |
