8G47
KRAS G12C complex with GDP and AMG 510 imaged on a cryo-EM imaging scaffold
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | RCG-33 - Cryo-EM imaging scaffold subunit B fused to DARPin | polymer | 321 | 35452.4 | 1 | synthetic construct | ||
| 2 | B (B) | GTPase KRas | polymer | 189 | 21622.3 | 1 | UniProt (P01116) Pfam (PF00071) | Homo sapiens (human) | K-Ras 2,Ki-Ras,c-K-ras,c-Ki-ras |
| 3 | C (B) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 4 | D (B) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) | |||
| 5 | E (B) | AMG 510 (bound form) | non-polymer | 562.6 | 1 | Chemie (MOV) |
Sequence modifications
B: 1 - 169 (UniProt: P01116)
| PDB | External Database | Details |
|---|---|---|
| Met -19 | - | expression tag |
| Gly -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Ser -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Ser -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Glu -6 | - | expression tag |
| Asn -5 | - | expression tag |
| Leu -4 | - | expression tag |
| Tyr -3 | - | expression tag |
| Phe -2 | - | expression tag |
| Gln -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Cys 12 | Gly 12 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 57074.7 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 1030.1 | |
| All* | Total formula weight | 58104.8 |
