8G2Y

Cryo-EM structure of ADGRF1 coupled to miniGs/q

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	MiniG alpha s/q chimera	polymer	423	47835.9	1		Homo sapiens (human)
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39418.1	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (N)	Nanobody 35	polymer	181	19726.2	1		Lama glama
5	E (R)	Adhesion G-protein-coupled receptor F1	polymer	411	45837.5	1	UniProt (A0A0C4DH10) Pfam (PF00002) UniProt (by SIFTS) (Q5T601)	Homo sapiens (human)
6	F (R)	[1-MYRISTOYL-GLYCEROL-3-YL]PHOSPHONYLCHOLINE	non-polymer		468.6	1	Chemie (LPC)

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

R: 567 - 910 (UniProt: A0A0C4DH10)

PDB	External Database	Details
Met 523	-	initiating methionine
Lys 524	-	expression tag
Thr 525	-	expression tag
Ile 526	-	expression tag
Ile 527	-	expression tag
Ala 528	-	expression tag
Leu 529	-	expression tag
Ser 530	-	expression tag
Tyr 531	-	expression tag
Ile 532	-	expression tag
Phe 533	-	expression tag
Cys 534	-	expression tag
Leu 535	-	expression tag
Val 536	-	expression tag
Phe 537	-	expression tag
Ala 538	-	expression tag
Asp 539	-	expression tag
Tyr 540	-	expression tag
Lys 541	-	expression tag
Asp 542	-	expression tag
His 543	-	expression tag
Asp 544	-	expression tag
Gly 545	-	expression tag
Asp 546	-	expression tag
Tyr 547	-	expression tag
Lys 548	-	expression tag
Asp 549	-	expression tag
His 550	-	expression tag
Asp 551	-	expression tag
Ile 552	-	expression tag
Asp 553	-	expression tag
Tyr 554	-	expression tag
Lys 555	-	expression tag
Asp 556	-	expression tag
Asp 557	-	expression tag
Asp 558	-	expression tag
Asp 559	-	expression tag
Lys 560	-	expression tag
Glu 561	-	expression tag
Asn 562	-	expression tag
Leu 563	-	expression tag
Tyr 564	-	expression tag
Phe 565	-	expression tag
Gln 566	-	expression tag
Gly 911	-	expression tag
Gly 912	-	expression tag
Gly 913	-	expression tag
Gly 914	-	expression tag
Ser 915	-	expression tag
Gly 916	-	expression tag
Gly 917	-	expression tag
Gly 918	-	expression tag
Gly 919	-	expression tag
Ser 920	-	expression tag
Ser 921	-	expression tag
Gly 922	-	expression tag
Val 923	-	expression tag
Thr 924	-	expression tag
Gly 925	-	expression tag
Tyr 926	-	expression tag
Arg 927	-	expression tag
Leu 928	-	expression tag
Phe 929	-	expression tag
Glu 930	-	expression tag
Glu 931	-	expression tag
Ile 932	-	expression tag
Leu 933	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	160678.9
Non-Polymers*	Number of molecules	1
	Total formula weight	468.6
All*	Total formula weight	161147.4

*Water molecules are not included.