8G2W
Cryo-EM structure of 3DVA component 2 of Escherichia coli que-PEC (paused elongation complex) RNA Polymerase minus preQ1 ligand
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA (39-MER) | polymer | 39 | 12063.8 | 1 | Escherichia coli | ||
| 2 | B (B) | DNA (31-MER) | polymer | 31 | 9486.1 | 1 | Escherichia coli | ||
| 3 | C, D (G, H) | DNA-directed RNA polymerase subunit alpha | polymer | 235 | 25971.5 | 2 | UniProt (A0A5B9AW69) Pfam (PF01193) Pfam (PF01000) UniProt (by SIFTS) (P0A7Z4) | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 4 | E (I) | DNA-directed RNA polymerase subunit beta | polymer | 1340 | 150560.6 | 1 | UniProt (C3SIA7) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (P0A8V2) | Escherichia coli | |
| 5 | F (J) | DNA-directed RNA polymerase subunit beta' | polymer | 1358 | 150436.3 | 1 | UniProt (C3SIA2) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) | Escherichia coli | |
| 6 | G (K) | DNA-directed RNA polymerase subunit omega | polymer | 79 | 8963.0 | 1 | UniProt (P0A800) Pfam (PF01192) | Escherichia coli | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 7 | H (R) | RNA (47-MER) | polymer | 47 | 15059.1 | 1 | Escherichia coli | ||
| 8 | I (J) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
G, H: 1 - 234 (UniProt: A0A5B9AW69)
| PDB | External Database | Details |
|---|---|---|
| Glu 235 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 398511.9 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 24.3 | |
| All* | Total formula weight | 398536.2 |
