8FVU

Cryo-EM structure of human Needle/NAIP/NLRC4 (R288A)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Baculoviral IAP repeat-containing protein 1	polymer	1409	160484.4	1	UniProt (Q13075) Pfam (PF00653) Pfam (PF05729) Pfam (PF22524) Pfam (PF17889)	Homo sapiens (human)	Neuronal apoptosis inhibitory protein
2	B (B)	NLR family CARD domain-containing protein 4	polymer	1030	116941.5	1	UniProt (Q9NPP4) Pfam (PF17889) Pfam (PF00619) Pfam (PF05729) Pfam (PF22524)	Homo sapiens (human)	CARD,LRR,and NACHT-containing protein,CED-4-like protein Clan,Caspase recruitment domain-containing protein 12,Ice protease-activating factor,Ipaf
3	C (U)	Lethal factor,Type III secretion system protein	polymer	374	42830.8	1	UniProt (P15917) UniProt (Q63K18) Pfam (PF07737) Pfam (PF09392)	Bacillus anthracis	LF,Anthrax lethal toxin endopeptidase component
4	D (A)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	1	Chemie (ATP)
5	E, F, G (A)	ZINC ION	non-polymer		65.4	3	Chemie (ZN)

Sequence modifications

A: 1 - 1403 (UniProt: Q13075)

PDB	External Database	Details
Ala -5	-	expression tag
Glu -4	-	expression tag
Tyr -3	-	expression tag
Cys -2	-	expression tag
Phe -1	-	expression tag
Asn 0	-	expression tag

B: 1 - 1024 (UniProt: Q9NPP4)

PDB	External Database	Details
Ala -5	-	expression tag
Glu -4	-	expression tag
Tyr -3	-	expression tag
Cys -2	-	expression tag
Phe -1	-	expression tag
Asn 0	-	expression tag
Ala 288	Arg 288	engineered mutation

U: -283 - -21 (UniProt: P15917)

PDB	External Database	Details
Gly -303	-	expression tag
Ser -302	-	expression tag
Ser -301	-	expression tag
His -300	-	expression tag
His -299	-	expression tag
His -298	-	expression tag
His -297	-	expression tag
His -296	-	expression tag
His -295	-	expression tag
Ser -294	-	expression tag
Ser -293	-	expression tag
Gly -292	-	expression tag
Leu -291	-	expression tag
Val -290	-	expression tag
Pro -289	-	expression tag
Arg -288	-	expression tag
Gly -287	-	expression tag
Ser -286	-	expression tag
His -285	-	expression tag
Met -284	-	expression tag
Ser -20	-	linker
Thr -19	-	linker
Arg -18	-	linker

U: -17 - 70 (UniProt: Q63K18)

PDB	External Database	Details
Thr -9	Ala 10	conflict
Thr 8	Asp 27	conflict
Gln 15	Lys 34	conflict

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	320256.7
Non-Polymers*	Number of molecules	4
	Total formula weight	703.4
All*	Total formula weight	320960.1

*Water molecules are not included.