8FVU
Cryo-EM structure of human Needle/NAIP/NLRC4 (R288A)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Baculoviral IAP repeat-containing protein 1 | polymer | 1409 | 160484.4 | 1 | UniProt (Q13075) Pfam (PF00653) Pfam (PF05729) Pfam (PF22524) Pfam (PF17889) In PDB | Homo sapiens (human) | Neuronal apoptosis inhibitory protein |
2 | B | NLR family CARD domain-containing protein 4 | polymer | 1030 | 116941.5 | 1 | UniProt (Q9NPP4) Pfam (PF17889) Pfam (PF00619) Pfam (PF05729) Pfam (PF22524) In PDB | Homo sapiens (human) | CARD,LRR,and NACHT-containing protein,CED-4-like protein Clan,Caspase recruitment domain-containing protein 12,Ice protease-activating factor,Ipaf |
3 | U | Lethal factor,Type III secretion system protein | polymer | 374 | 42830.8 | 1 | UniProt (P15917) UniProt (Q63K18) Pfam (PF07737) Pfam (PF09392) In PDB | Bacillus anthracis | LF,Anthrax lethal toxin endopeptidase component |
4 | A | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 1 | Chemie (ATP) | |||
5 | A | ZINC ION | non-polymer | 65.4 | 3 | Chemie (ZN) |
Sequence modifications
A: 1 - 1403 (UniProt: Q13075)
B: 1 - 1024 (UniProt: Q9NPP4)
U: -283 - -21 (UniProt: P15917)
U: -17 - 70 (UniProt: Q63K18)
PDB | External Database | Details |
---|---|---|
Ala -5 | - | expression tag |
Glu -4 | - | expression tag |
Tyr -3 | - | expression tag |
Cys -2 | - | expression tag |
Phe -1 | - | expression tag |
Asn 0 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Ala -5 | - | expression tag |
Glu -4 | - | expression tag |
Tyr -3 | - | expression tag |
Cys -2 | - | expression tag |
Phe -1 | - | expression tag |
Asn 0 | - | expression tag |
Ala 288 | Arg 288 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Gly -303 | - | expression tag |
Ser -302 | - | expression tag |
Ser -301 | - | expression tag |
His -300 | - | expression tag |
His -299 | - | expression tag |
His -298 | - | expression tag |
His -297 | - | expression tag |
His -296 | - | expression tag |
His -295 | - | expression tag |
Ser -294 | - | expression tag |
Ser -293 | - | expression tag |
Gly -292 | - | expression tag |
Leu -291 | - | expression tag |
Val -290 | - | expression tag |
Pro -289 | - | expression tag |
Arg -288 | - | expression tag |
Gly -287 | - | expression tag |
Ser -286 | - | expression tag |
His -285 | - | expression tag |
Met -284 | - | expression tag |
Ser -20 | - | linker |
Thr -19 | - | linker |
Arg -18 | - | linker |
PDB | External Database | Details |
---|---|---|
Thr -9 | Ala 10 | conflict |
Thr 8 | Asp 27 | conflict |
Gln 15 | Lys 34 | conflict |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 320256.7 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 703.4 | |
All* | Total formula weight | 320960.1 |