8FTU

Crystal structure of the SNARE Use1 bound to Dsl1 complex subunits Sec39 and Dsl1, Revised Use1 structure

Replaces:  6WC4

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Protein transport protein SEC39	polymer	699	79860.9	1	UniProt (Q6CWC7) Pfam (PF08314) In PDB	Kluyveromyces lactis NRRL Y-1140
2	B	Vesicle transport protein USE1	polymer	113	13260.6	1	UniProt (Q6CL08) Pfam (PF09753) In PDB	Kluyveromyces lactis NRRL Y-1140
3	C	Protein transport protein DSL1	polymer	306	36014.4	1	UniProt (Q6CUS2) Pfam (PF11989) In PDB	Kluyveromyces lactis NRRL Y-1140

Sequence modifications

A: 1 - 672 (UniProt: Q6CWC7)

PDB	External Database	Details
Met -26	-	expression tag
Lys -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
Gly -17	-	expression tag
Ala -16	-	expression tag
Ala -15	-	expression tag
Gly -14	-	expression tag
Thr -13	-	expression tag
Ser -12	-	expression tag
Leu -11	-	expression tag
Tyr -10	-	expression tag
Lys -9	-	expression tag
Lys -8	-	expression tag
Ala -7	-	expression tag
Gly -6	-	expression tag
Leu -5	-	expression tag
Val -4	-	expression tag
Pro -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

B: 4 - 113 (UniProt: Q6CL08)

PDB	External Database	Details
Met 1	-	expression tag
Gly 2	-	expression tag
Ser 3	-	expression tag

C: 422 - 456 (UniProt: Q6CUS2)

PDB	External Database	Details
Met 419	-	expression tag
Gly 420	-	expression tag
Ser 421	-	expression tag

C: 514 - 776 (UniProt: Q6CUS2)

PDB	External Database	Details
Gly 509	-	linker
Asp 510	-	linker
Gly 511	-	linker
Asp 512	-	linker
Gly 513	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	129135.9
All*	Total formula weight	129135.9

*Water molecules are not included.