8FTU

Crystal structure of the SNARE Use1 bound to Dsl1 complex subunits Sec39 and Dsl1, Revised Use1 structure

Replaces:  6WC4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97932
Spacegroup name	C 1 2 1
Unit cell lengths	240.790, 87.846, 161.431
Unit cell angles	90.00, 107.61, 90.00

Refinement procedure

Resolution	29.600 - 5.730
R-factor	0.2782
Rwork	0.276
R-free	0.29800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.856
Data reduction software	XDS
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.17.1-3660-000)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.601	29.601	6.645
High resolution limit [Å]	5.726	12.006	5.726
Rmerge	0.115	0.052	1.814
Rmeas	0.135	0.061	2.171
Rpim	0.070	0.032	1.184
Number of reflections	7053	1007	1008
<I/σ(I)>	10.7	26.4	1.1
Completeness [%]	74.7	100	29.8
Redundancy	6.8	6.7	6.4
CC(1/2)	0.997	0.997	0.319

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	0.1 M Tris, pH 7.5, 0.1 M NaCl, 8% (w/v) PEG 3350, 10% (v/v) ethylene glycol, 1 mM dithiothreitol. Cryoprotected with 30% (v/v) ethylene glycol