8FSJ

Cryo-EM structure of engineered hepatitis C virus E1E2 ectodomain in complex with antibodies AR4A, HEPC74, and IGH520

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	E	HCV E2 ectodomain, SYNZIP2 scaffold fusion	polymer	385	42975.4	1	UniProt (A0A2P0NE15) Pfam (PF01560) In PDB	Hepacivirus C
2	H	HEPC74 heavy chain	polymer	264	27644.8	1		Homo sapiens (human)
3	L	HEPC74 light chain	polymer	214	23470.1	1		Homo sapiens (human)
4	A	HCV E1 ectodomain, SYNZIP1 scaffold fusion	polymer	206	22951.4	1	UniProt (A0A2P0NE34) Pfam (PF01539) UniProt (by SIFTS) (Q81387) In PDB	Hepacivirus C
5	B	AR4A light chain	polymer	236	25743.8	1		Homo sapiens (human)
6	C	AR4A heavy chain	polymer	298	31862.7	1		Homo sapiens (human)
7	D, F	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
8	G, I, J, K, M...	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	6	In PDB GlyTouCan (G15407YE)
9	E, A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)

Sequence modifications

E: 384 - 714 (UniProt: A0A2P0NE15)
E: 715 - 768 (PDB: 8FSJ)
A: 192 - 349 (UniProt: A0A2P0NE34)
A: 350 - 397 (PDB: 8FSJ)

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	174648.1
Branched	Number of molecules	8
	Total formula weight	4368.1
Non-Polymers*	Number of molecules	6
	Total formula weight	1327.2
All*	Total formula weight	180343.4

*Water molecules are not included.