8FSD

P130R mutant of soybean SHMT8 in complex with PLP-glycine and formylTHF

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Serine hydroxymethyltransferase	polymer	491	54063.3	4	UniProt (A0A0R0IK90) Pfam (PF00464)	Glycine max (soybean)
2	E, FA, O, V (A, D, B, C)	N-GLYCINE-[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YL-METHANE]	non-polymer		306.2	4	Chemie (PLG) PubChem (445062)
3	AA, BA, DA, EA, F... (C, D, A, B)	1,2-ETHANEDIOL	non-polymer		62.1	26	Chemie (EDO)
4	CA, KA, L, U (C, D, A, B)	N-[4-({[(6S)-2-amino-5-formyl-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)benzoyl]-L-glutamic acid	non-polymer		473.4	4	Chemie (FFO) PubChem (135398559) PubChem (135402009) PubChem (135403648) PubChem (135445225) PubChem (135481887) PubChem (135484651) PubChem (135665655) PubChem (135871200) PubChem (136123990)
5	MA, NA, OA, PA (A, B, C, D)	water	water		18.0	2017	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 471 (UniProt: A0A0R0IK90)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Arg 130	Pro 200	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	216253.2
Non-Polymers*	Number of molecules	34
	Total formula weight	4732.4
All*	Total formula weight	220985.5

*Water molecules are not included.