8FN1
CryoEM structure of Go-coupled NTSR1
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | F | Neurotensin/neuromedin N | polymer | 6 | 819.0 | 1 | UniProt (P20068) In PDB | Rattus norvegicus | |
2 | A | Neurotensin receptor type 1 | polymer | 408 | 45842.1 | 1 | UniProt (P20789) Pfam (PF00001) In PDB | Rattus norvegicus (Norway rat) | NT-R-1,NTR1,High-affinity levocabastine-insensitive neurotensin receptor,NTRH |
3 | B | Guanine nucleotide-binding protein G(o) subunit alpha | polymer | 228 | 25451.2 | 1 | UniProt (P09471) Pfam (PF00503) In PDB | Spodoptera frugiperda (fall armyworm) | |
4 | C | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 358 | 39418.1 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (human) | Transducin beta chain 1 |
5 | D | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (human) | G gamma-I |
6 | E | scFv16 | polymer | 267 | 28668.9 | 1 | Escherichia coli |
Sequence modifications
A: 39 - 424 (UniProt: P20789)
B: -2 - 54 (UniProt: P09471)
B: 63 - 225 (UniProt: P09471)
C: 2 - 340 (UniProt: P62873)
PDB | External Database | Details |
---|---|---|
Met 17 | - | expression tag |
His 18 | - | expression tag |
His 19 | - | expression tag |
His 20 | - | expression tag |
His 21 | - | expression tag |
His 22 | - | expression tag |
His 23 | - | expression tag |
His 24 | - | expression tag |
His 25 | - | expression tag |
His 26 | - | expression tag |
His 27 | - | expression tag |
Ser 28 | - | expression tag |
Asp 29 | - | expression tag |
Leu 30 | - | expression tag |
Glu 31 | - | expression tag |
Val 32 | - | expression tag |
Leu 33 | - | expression tag |
Phe 34 | - | expression tag |
Gln 35 | - | expression tag |
Gly 36 | - | expression tag |
Pro 37 | - | expression tag |
Leu 38 | - | expression tag |
Ala 42 | Asn 42 | conflict |
Leu 86 | Ala 86 | conflict |
Ala 215 | Gly 215 | conflict |
Ala 360 | Val 360 | conflict |
PDB | External Database | Details |
---|---|---|
Asp 6 | Glu 9 | conflict |
Lys 7 | Arg 10 | conflict |
Val 10 | Leu 13 | conflict |
Met 15 | Ala 18 | conflict |
Asp 39 | Gly 42 | conflict |
Asn 40 | Glu 43 | conflict |
PDB | External Database | Details |
---|---|---|
Gly 55 | - | linker |
Gly 56 | - | linker |
Ser 57 | - | linker |
Gly 58 | - | linker |
Gly 59 | - | linker |
Ser 60 | - | linker |
Gly 61 | - | linker |
Gly 62 | - | linker |
Asp 108 | Ala 227 | conflict |
Asp 111 | Gly 230 | conflict |
- | Asp 232 | deletion |
- | Gln 233 | deletion |
- | Val 234 | deletion |
- | Leu 235 | deletion |
- | His 236 | deletion |
- | Glu 237 | deletion |
- | Asp 238 | deletion |
- | Glu 239 | deletion |
- | Thr 240 | deletion |
- | Thr 241 | deletion |
Ala 203 | Ile 332 | conflict |
Ile 206 | Val 335 | conflict |
PDB | External Database | Details |
---|---|---|
Met -17 | - | expression tag |
His -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
Leu -10 | - | expression tag |
Glu -9 | - | expression tag |
Val -8 | - | expression tag |
Leu -7 | - | expression tag |
Phe -6 | - | expression tag |
Gln -5 | - | expression tag |
Gly -4 | - | expression tag |
Pro -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
Ser 0 | - | expression tag |
Gly 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 148060.4 | |
All* | Total formula weight | 148060.4 |