8FN1

CryoEM structure of Go-coupled NTSR1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (F)	Neurotensin/neuromedin N	polymer	6	819.0	1	UniProt (P20068)	Rattus norvegicus
2	B (A)	Neurotensin receptor type 1	polymer	408	45842.1	1	UniProt (P20789) Pfam (PF00001)	Rattus norvegicus (Norway rat)	NT-R-1,NTR1,High-affinity levocabastine-insensitive neurotensin receptor,NTRH
3	C (B)	Guanine nucleotide-binding protein G(o) subunit alpha	polymer	228	25451.2	1	UniProt (P09471) Pfam (PF00503)	Spodoptera frugiperda (fall armyworm)
4	D (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39418.1	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
5	E (D)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
6	F (E)	scFv16	polymer	267	28668.9	1		Escherichia coli

Sequence modifications

A: 39 - 424 (UniProt: P20789)

PDB	External Database	Details
Met 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag
His 24	-	expression tag
His 25	-	expression tag
His 26	-	expression tag
His 27	-	expression tag
Ser 28	-	expression tag
Asp 29	-	expression tag
Leu 30	-	expression tag
Glu 31	-	expression tag
Val 32	-	expression tag
Leu 33	-	expression tag
Phe 34	-	expression tag
Gln 35	-	expression tag
Gly 36	-	expression tag
Pro 37	-	expression tag
Leu 38	-	expression tag
Ala 42	Asn 42	conflict
Leu 86	Ala 86	conflict
Ala 215	Gly 215	conflict
Ala 360	Val 360	conflict

B: -2 - 54 (UniProt: P09471)

PDB	External Database	Details
Asp 6	Glu 9	conflict
Lys 7	Arg 10	conflict
Val 10	Leu 13	conflict
Met 15	Ala 18	conflict
Asp 39	Gly 42	conflict
Asn 40	Glu 43	conflict

B: 63 - 225 (UniProt: P09471)

PDB	External Database	Details
Gly 55	-	linker
Gly 56	-	linker
Ser 57	-	linker
Gly 58	-	linker
Gly 59	-	linker
Ser 60	-	linker
Gly 61	-	linker
Gly 62	-	linker
Asp 108	Ala 227	conflict
Asp 111	Gly 230	conflict
-	Asp 232	deletion
-	Gln 233	deletion
-	Val 234	deletion
-	Leu 235	deletion
-	His 236	deletion
-	Glu 237	deletion
-	Asp 238	deletion
-	Glu 239	deletion
-	Thr 240	deletion
-	Thr 241	deletion
Ala 203	Ile 332	conflict
Ile 206	Val 335	conflict

C: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	148060.4
All*	Total formula weight	148060.4

*Water molecules are not included.