8FLT

Human PTH1R in complex with M-PTH(1-14) and Gs

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45683.4	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37413.9	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	58	6375.3	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (N)	Nanobody35	polymer	128	13885.4	1		Lama glama (llama)
5	E (P)	M-PTH(1-14)	polymer	14	1725.1	1		synthetic construct
6	F (R)	Parathyroid hormone/parathyroid hormone-related peptide receptor	polymer	616	69513.8	1	UniProt (Q03431) Pfam (PF02793) Pfam (PF00002)	Homo sapiens (human)	PTH/PTHrP type I receptor,PTH/PTHr receptor,Parathyroid hormone 1 receptor,PTH1 receptor

Sequence modifications

A: 1 - 394 (UniProt: P63092)

PDB	External Database	Details
Asn 54	Ser 54	engineered mutation
Ala 226	Gly 226	engineered mutation
Ala 268	Glu 268	engineered mutation
Lys 271	Asn 271	engineered mutation
Asp 274	Lys 274	engineered mutation
Lys 280	Arg 280	engineered mutation
Asp 284	Thr 284	engineered mutation
Thr 285	Ile 285	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Gln 1	-	expression tag

R: 28 - 593 (UniProt: Q03431)

PDB	External Database	Details
Met -3	-	expression tag
Lys -2	-	expression tag
Thr -1	-	expression tag
Ile 0	-	expression tag
Ile 1	-	expression tag
Ala 2	-	expression tag
Leu 3	-	expression tag
Ser 4	-	expression tag
Tyr 5	-	expression tag
Ile 6	-	expression tag
Phe 7	-	expression tag
Cys 8	-	expression tag
Leu 9	-	expression tag
Val 10	-	expression tag
Phe 11	-	expression tag
Ala 12	-	expression tag
Asp 13	-	expression tag
Tyr 14	-	expression tag
Lys 15	-	expression tag
Asp 16	-	expression tag
Asp 17	-	expression tag
Asp 18	-	expression tag
Asp 19	-	expression tag
Leu 20	-	expression tag
Glu 21	-	expression tag
Val 22	-	expression tag
Leu 23	-	expression tag
Phe 24	-	expression tag
Gln 25	-	expression tag
Gly 26	-	expression tag
Pro 27	-	expression tag
Pro 594	-	expression tag
Ala 595	-	expression tag
Gly 596	-	expression tag
Leu 597	-	expression tag
Glu 598	-	expression tag
Val 599	-	expression tag
Leu 600	-	expression tag
Phe 601	-	expression tag
Gln 602	-	expression tag
Gly 603	-	expression tag
Pro 604	-	expression tag
His 605	-	expression tag
His 606	-	expression tag
His 607	-	expression tag
His 608	-	expression tag
His 609	-	expression tag
His 610	-	expression tag
His 611	-	expression tag
His 612	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	174596.9
All*	Total formula weight	174596.9

*Water molecules are not included.