8FHW

Cryo-EM structure of Cryptococcus neoformans trehalose-6-phosphate synthase homotetramer in complex with uridine diphosphate and glucose-6-phosphate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Alpha,alpha-trehalose-phosphate synthase (UDP-forming)	polymer	693	76815.5	4	UniProt (J9VJZ1) Pfam (PF00982) In PDB	Cryptococcus neoformans var. grubii H99
2	A, B, C, D	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	4	Chemie (UDP)
3	A, B, C, D	6-O-phosphono-alpha-D-glucopyranose	non-polymer		260.1	4	Chemie (G6P)

Sequence modifications

A, B, C, D: 1 - 669 (UniProt: J9VJZ1)

PDB	External Database	Details
Met -23	-	initiating methionine
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
Gly -14	-	expression tag
Val -13	-	expression tag
Asp -12	-	expression tag
Leu -11	-	expression tag
Gly -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	307262.0
Non-Polymers*	Number of molecules	8
	Total formula weight	2657.2
All*	Total formula weight	309919.2

*Water molecules are not included.