8FHW

Cryo-EM structure of Cryptococcus neoformans trehalose-6-phosphate synthase homotetramer in complex with uridine diphosphate and glucose-6-phosphate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Alpha,alpha-trehalose-phosphate synthase (UDP-forming)	polymer	693	76815.5	4	UniProt (J9VJZ1)	Cryptococcus neoformans var. grubii H99
2	E, G, I, K (A, B, C, D)	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	4	Chemie (UDP) PubChem (1158) PubChem (6031) PubChem (25323044) PubChem (25323048) PubChem (44298965) PubChem (53486216) PubChem (3084486) PubChem (60098857) PubChem (57448939) PubChem (6101632) PubChem (6604175) PubChem (91746160) PubChem (92332560) PubChem (92332561) PubChem (92332562) PubChem (92332563) PubChem (11811538) PubChem (93088131) PubChem (93630083) PubChem (12049241)
3	F, H, J, L (A, B, C, D)	6-O-phosphono-alpha-D-glucopyranose	non-polymer		260.1	4	Chemie (G6P)

Sequence modifications

A, B, C, D: 1 - 669 (UniProt: J9VJZ1)

PDB	External Database	Details
Met -23	-	initiating methionine
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
Gly -14	-	expression tag
Val -13	-	expression tag
Asp -12	-	expression tag
Leu -11	-	expression tag
Gly -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	307262.0
Non-Polymers*	Number of molecules	8
	Total formula weight	2657.2
All*	Total formula weight	309919.2

*Water molecules are not included.