8FHO

Cryo-EM structure of human NCC (class 1)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Solute carrier family 12 member 2,Solute carrier family 12 member 3 chimera	polymer	1092	120372.2	3	UniProt (A0A0G2KTI4) UniProt (P55017) Pfam (PF08403) Pfam (PF00324) Pfam (PF03522) In PDB	Danio rerio (Zebrafish Brachydanio rerio)	Na-K-Cl cotransporter 1,Na-Cl cotransporter,NCC,Na-Cl symporter,Thiazide-sensitive sodium-chloride cotransporter
2	A, B	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
3	A, B	Polythiazide	non-polymer		439.9	2	Chemie (XZF)
4	A, B	SODIUM ION	non-polymer		23.0	2	Chemie (NA)

Sequence modifications

A, B, C: -70 - 131 (UniProt: A0A0G2KTI4)

PDB	External Database	Details
Lys 5	Glu 76	conflict

A, B, C: null - 1021 (UniProt: P55017)

PDB	External Database	Details
Ala 240	Glu 240	engineered mutation
Gly 264	Ala 264	conflict

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	361116.5
Non-Polymers*	Number of molecules	6
	Total formula weight	1940.1
All*	Total formula weight	363056.6

*Water molecules are not included.