8FFZ

TFIIIA-TFIIIC-Brf1-TBP complex bound to 5S rRNA gene

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Transcription factor IIIA	polymer	451	52522.5	1	UniProt (P39933) Pfam (PF00096) Pfam (PF13894) In PDB	Saccharomyces cerevisiae (baker's yeast)	TFIIIA,Putative zinc finger protein 1
2	B	Transcription factor tau 138 kDa subunit	polymer	1160	132313.1	1	UniProt (P34111) Pfam (PF04182) Pfam (PF21552) Pfam (PF20222) In PDB	Saccharomyces cerevisiae (baker's yeast)	TFIIIC 138 kDa subunit,Transcription factor C subunit 3
3	C	Transcription factor tau 131 kDa subunit	polymer	1025	120388.6	1	UniProt (P33339) Pfam (PF13432) Pfam (PF13181) In PDB	Saccharomyces cerevisiae (baker's yeast)	TFIIIC 131 kDa subunit,Transcription factor C subunit 4
4	D	Transcription factor tau 95 kDa subunit	polymer	649	73649.4	1	UniProt (P32367) Pfam (PF09734) Pfam (PF17682) In PDB	Saccharomyces cerevisiae (baker's yeast)	TFIIIC 95 kDa subunit,Transcription factor C subunit 1
5	E	Transcription factor tau 91 kDa subunit	polymer	672	74803.3	1	UniProt (Q06339) In PDB	Saccharomyces cerevisiae (baker's yeast)	TFIIIC 91 kDa subunit,Transcription factor C subunit 6
6	F	Transcription factor tau 60 kDa subunit	polymer	588	67755.2	1	UniProt (Q12308) Pfam (PF12660) In PDB	Saccharomyces cerevisiae (baker's yeast)	TFIIIC 60 kDa subunit,Transcription factor C subunit 8
7	G	Transcription factor tau 55 kDa subunit	polymer	435	49198.9	1	UniProt (Q12415) Pfam (PF00300) Pfam (PF10419) In PDB	Saccharomyces cerevisiae (baker's yeast)	TFIIIC 55 kDa subunit,Transcription factor C subunit 7
8	H	Transcription factor IIIB 70 kDa subunit,TATA-box-binding protein	polymer	736	82097.9	1	UniProt (P29056) UniProt (P13393) Pfam (PF08271) Pfam (PF00382) Pfam (PF00352) Pfam (PF07741) In PDB	Saccharomyces cerevisiae S288C	TFIIIB,B-related factor 1,BRF-1,TATA sequence-binding protein,TBP,TATA-binding factor,TATA-box factor,Transcription factor D,Transcription initiation factor TFIID TBP subunit
9	I	DNA (151-MER)	polymer	171	52636.6	1	GenBank (834774822)	Saccharomyces cerevisiae
10	J	DNA (151-MER)	polymer	171	52934.8	1	GenBank (834774822)	Saccharomyces cerevisiae
11	A	ZINC ION	non-polymer		65.4	9	Chemie (ZN)

Sequence modifications

A: 1 - 429 (UniProt: P39933)

PDB	External Database	Details
Ala 430	-	expression tag
Asp 431	-	expression tag
Pro 432	-	expression tag
Asn 433	-	expression tag
Ser 434	-	expression tag
Ser 435	-	expression tag
Ser 436	-	expression tag
Val 437	-	expression tag
Asp 438	-	expression tag
Lys 439	-	expression tag
Leu 440	-	expression tag
Ala 441	-	expression tag
Ala 442	-	expression tag
Ala 443	-	expression tag
Leu 444	-	expression tag
Glu 445	-	expression tag
His 446	-	expression tag
His 447	-	expression tag
His 448	-	expression tag
His 449	-	expression tag
His 450	-	expression tag
His 451	-	expression tag

H: 1 - 382 (UniProt: P29056)
H: 387 - 566 (UniProt: P13393)

PDB	External Database	Details
Gly 567	-	linker
Ser 568	-	linker
Gly 569	-	linker
Ser 570	-	linker
Gly 571	-	linker
Ser 572	-	linker
Gly 573	-	linker
Ser 574	-	linker
Gly 575	-	linker
Ser 576	-	linker
Gly 577	-	linker
Ser 578	-	linker

H: 579 - 736 (UniProt: P29056)

PDB	External Database	Details
Ala 383	-	linker
Met 384	-	linker
Pro 385	-	linker
Trp 386	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	758300.2
Non-Polymers*	Number of molecules	9
	Total formula weight	588.7
All*	Total formula weight	758888.9

*Water molecules are not included.