8FEG
CryoEM structure of Kappa Opioid Receptor bound to a semi-peptide and Gi1
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (C) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40414.0 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 2 | B (D) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 358 | 39418.1 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 3 | C (E) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 4 | D (F) | scFv16 antibody fragment | polymer | 251 | 26679.7 | 1 | Mus musculus (Mouse) | ||
| 5 | E (B) | Kappa-type opioid receptor | polymer | 308 | 34944.6 | 1 | UniProt (P41145) Pfam (PF00001) | Homo sapiens (human) | K-OR-1,KOR-1 |
| 6 | F (A) | ACE-TYR-ALA-DTY-THR-THR-CYS-THR-DPN-XT9 | polymer | 10 | 1307.5 | 1 | synthetic construct | ||
| 7 | G (B) | water | water | 18.0 | 2 | Chemie (HOH) |
Sequence modifications
C: 1 - 354 (UniProt: P63096)
D: 2 - 340 (UniProt: P62873)
B: 54 - 358 (UniProt: P41145)
| PDB | External Database | Details |
|---|---|---|
| Asn 47 | Ser 47 | engineered mutation |
| Ala 203 | Gly 203 | engineered mutation |
| Ala 245 | Glu 245 | engineered mutation |
| Ser 326 | Ala 326 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| Leu -10 | - | expression tag |
| Glu -9 | - | expression tag |
| Val -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Phe -6 | - | expression tag |
| Gln -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Leu 51 | - | expression tag |
| Gly 52 | - | expression tag |
| Ser 53 | - | expression tag |
| Leu 135 | Ile 135 | conflict |
| Cys 324 | Ser 324 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 150625.1 | |
| All* | Total formula weight | 150625.1 |
