8FEG
CryoEM structure of Kappa Opioid Receptor bound to a semi-peptide and Gi1
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | C | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40414.0 | 1 | UniProt (P63096) Pfam (PF00503) In PDB | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
2 | D | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 358 | 39418.1 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (human) | Transducin beta chain 1 |
3 | E | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (human) | G gamma-I |
4 | F | scFv16 antibody fragment | polymer | 251 | 26679.7 | 1 | Mus musculus (Mouse) | ||
5 | B | Kappa-type opioid receptor | polymer | 308 | 34944.6 | 1 | UniProt (P41145) Pfam (PF00001) In PDB | Homo sapiens (human) | K-OR-1,KOR-1 |
6 | A | ACE-TYR-ALA-DTY-THR-THR-CYS-THR-DPN-XT9 | polymer | 10 | 1307.5 | 1 | synthetic construct | ||
7 | water | water | 18.0 | 2 | Chemie (HOH) |
Sequence modifications
C: 1 - 354 (UniProt: P63096)
D: 2 - 340 (UniProt: P62873)
B: 54 - 358 (UniProt: P41145)
PDB | External Database | Details |
---|---|---|
Asn 47 | Ser 47 | engineered mutation |
Ala 203 | Gly 203 | engineered mutation |
Ala 245 | Glu 245 | engineered mutation |
Ser 326 | Ala 326 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Met -17 | - | expression tag |
His -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
Leu -10 | - | expression tag |
Glu -9 | - | expression tag |
Val -8 | - | expression tag |
Leu -7 | - | expression tag |
Phe -6 | - | expression tag |
Gln -5 | - | expression tag |
Gly -4 | - | expression tag |
Pro -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
Ser 0 | - | expression tag |
Gly 1 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Leu 51 | - | expression tag |
Gly 52 | - | expression tag |
Ser 53 | - | expression tag |
Leu 135 | Ile 135 | conflict |
Cys 324 | Ser 324 | conflict |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 150625.1 | |
All* | Total formula weight | 150625.1 |