8FDT

Engineered human dynein motor domain in the microtubule-unbound state with LIS1 complex in the buffer containing ATP-Vi

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Cytoplasmic dynein 1 heavy chain 1,Serine--tRNA ligase	polymer	3126	357459.1	1	UniProt (Q14204) UniProt (Q5SJX7) Pfam (PF08393) Pfam (PF03028) Pfam (PF18198) Pfam (PF18199) Pfam (PF12774) Pfam (PF17852) Pfam (PF12775) Pfam (PF22597) Pfam (PF12780) Pfam (PF12777) Pfam (PF02403) Pfam (PF12781)	Homo sapiens (human)	Cytoplasmic dynein heavy chain 1,Dynein heavy chain,cytosolic,Seryl-tRNA synthetase,SerRS,Seryl-tRNA(Ser/Sec) synthetase
2	B, C (B, C)	Platelet-activating factor acetylhydrolase IB subunit beta	polymer	598	66423.5	2	UniProt (P43034) Pfam (PF08513) Pfam (PF00400)	Homo sapiens (human)	Lissencephaly-1 protein,LIS-1,PAF acetylhydrolase 45 kDa subunit,PAF-AH 45 kDa subunit,PAF-AH alpha,PAFAH alpha
3	D (A)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	1	Chemie (ATP)
4	E, F, G (A)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	3	Chemie (ADP)
5	H (A)	VANADATE ION	non-polymer		114.9	1	Chemie (VO4)

Sequence modifications

A: 3 - 1822 (UniProt: Q14204)

PDB	External Database	Details
Gly 1	-	expression tag
Ser 2	-	expression tag

A: 1823 - 1889 (UniProt: Q5SJX7)
A: 1890 - 3124 (UniProt: Q14204)

PDB	External Database	Details
Glu 3125	-	expression tag
Phe 3126	-	expression tag

B, C: 3 - 411 (UniProt: P43034)

PDB	External Database	Details
Gly 1	-	expression tag
Ser 2	-	expression tag

B, C: 412 - 598 (PDB: 8FDT)

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	490306.2
Non-Polymers*	Number of molecules	5
	Total formula weight	1903.7
All*	Total formula weight	492209.9

*Water molecules are not included.