8FC9

Cryo-EM structure of the human TRPV4 - RhoA, apo condition

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, F, G, H (A, B, C, D)	Transient receptor potential cation channel subfamily V member 4	polymer	901	102057.8	4	UniProt (Q9HBA0) Pfam (PF00023) Pfam (PF00520)	Homo sapiens (human)	TrpV4,Osm-9-like TRP channel 4,OTRPC4,Transient receptor potential protein 12,TRP12,Vanilloid receptor-like channel 2,Vanilloid receptor-like protein 2,VRL-2,Vanilloid receptor-related osmotically-activated channel,VR-OAC
2	B, C, D, E (E, F, G, H)	Transforming protein RhoA	polymer	193	21799.2	4	UniProt (P61586) Pfam (PF00071)	Homo sapiens (human)	Rho cDNA clone 12,h12
3	I, K, M, O (E, F, G, H)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
4	J, L, N, P (E, F, G, H)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	4	Chemie (GDP)

Sequence modifications

A, B, C, D: 1 - 871 (UniProt: Q9HBA0)

PDB	External Database	Details
Glu 872	-	expression tag
Asn 873	-	expression tag
Ser 874	-	expression tag
Leu 875	-	expression tag
Glu 876	-	expression tag
Val 877	-	expression tag
Leu 878	-	expression tag
Phe 879	-	expression tag
Gln 880	-	expression tag
Gly 881	-	expression tag
Pro 882	-	expression tag
Asp 883	-	expression tag
Tyr 884	-	expression tag
Lys 885	-	expression tag
Asp 886	-	expression tag
Asp 887	-	expression tag
Asp 888	-	expression tag
Asp 889	-	expression tag
Lys 890	-	expression tag
Ala 891	-	expression tag
His 892	-	expression tag
His 893	-	expression tag
His 894	-	expression tag
His 895	-	expression tag
His 896	-	expression tag
His 897	-	expression tag
His 898	-	expression tag
His 899	-	expression tag
His 900	-	expression tag
His 901	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	495427.8
Non-Polymers*	Number of molecules	8
	Total formula weight	1870.0
All*	Total formula weight	497297.8

*Water molecules are not included.