8FA1

Cryo-EM structure of the SARS-CoV-2 HR1HR2 fusion core complex with N969K mutation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Ferritin, Dps family protein and Spike protein S2' chimera	polymer	257	28774.1	3	UniProt (B2J981) UniProt (A0A8B6RKS7) Pfam (PF00210)	Nostoc punctiforme PCC 73102
2	D, E, F (D, E, F)	Spike protein S2' HR2	polymer	45	4935.4	3	UniProt (P0DTC2)	Severe acute respiratory syndrome coronavirus 2

Sequence modifications

A, B, C: 741 - 915 (UniProt: B2J981)

PDB	External Database	Details
Met 732	-	initiating methionine
Ser 733	-	expression tag
His 734	-	expression tag
His 735	-	expression tag
His 736	-	expression tag
His 737	-	expression tag
His 738	-	expression tag
His 739	-	expression tag
Gly 740	-	expression tag
Ser 741	Thr 4	conflict
Ala 916	-	linker

A, B, C: 917 - 988 (UniProt: A0A8B6RKS7)

PDB	External Database	Details
Lys 969	Asn 858	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	101128.7
All*	Total formula weight	101128.7

*Water molecules are not included.