8F7W

Gi bound kappa-opioid receptor in complex with dynorphin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Kappa-type opioid receptor	polymer	378	42434.7	1	UniProt (P41145) Pfam (PF00001)	Homo sapiens (human)	K-OR-1,KOR-1
2	B (P)	Dynorphin	polymer	8	983.2	1	UniProt (P01213)	Homo sapiens
3	C (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40445.1	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
4	D (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	353	39020.7	1	UniProt (P54311) Pfam (PF00400)	Rattus norvegicus (Norway rat)	Transducin beta chain 1
5	E (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	68	7563.8	1	UniProt (P63212) Pfam (PF00631)	Bos taurus (cattle)	G gamma-I
6	F (E)	scFv16	polymer	248	26408.5	1		synthetic construct
7	G, H, I (R)	CHOLESTEROL	non-polymer		386.7	3	Chemie (CLR)
8	J, K (R)	PALMITIC ACID	non-polymer		256.4	2	Chemie (PLM)

Sequence modifications

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Ala 203	Gly 203	conflict
Ser 326	Ala 326	conflict

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	156855.9
Non-Polymers*	Number of molecules	5
	Total formula weight	1672.8
All*	Total formula weight	158528.7

*Water molecules are not included.