8F7S

Gi bound delta-opioid receptor in complex with deltorphin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, F (R, D)	Delta-type opioid receptor	polymer	390	42651.0	2	UniProt (P41143) Pfam (PF00001)	Homo sapiens (human)	D-OR-1,DOR-1
2	B, G (P, Q)	deltorphin	polymer	8	781.9	2	UniProt (P21850)	Phyllomedusa	[D-Ala2]-deltorphin II
3	C, H (A, F)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40445.1	2	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
4	D (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	353	39020.7	1	UniProt (P54311) Pfam (PF00400)	Rattus norvegicus (Norway rat)	Transducin beta chain 1
5	E (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	68	7563.8	1	UniProt (P63212) Pfam (PF00631)	Bos taurus (cattle)	G gamma-I
6	I, J, T, U, V (R, D)	CHOLESTEROL	non-polymer		386.7	5	Chemie (CLR)
7	K, L, M, N, O... (R, D)	PALMITIC ACID	non-polymer		256.4	12	Chemie (PLM)

Sequence modifications

R, D: 2 - 372 (UniProt: P41143)

PDB	External Database	Details
Asp -9	-	expression tag
Tyr -8	-	expression tag
Lys -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Val -2	-	expression tag
Asp -1	-	expression tag
Ala 0	-	expression tag
Ser 1	-	expression tag
Val 73	Gly 73	conflict
Ser 90	Asn 90	conflict
Gly 95	Asp 95	conflict
Ser 131	Asn 131	conflict
Cys 143	Ser 143	conflict
Val 268	Gly 268	conflict
Ile 309	Ala 309	conflict
His 373	-	expression tag
His 374	-	expression tag
His 375	-	expression tag
His 376	-	expression tag
His 377	-	expression tag
His 378	-	expression tag
His 379	-	expression tag
His 380	-	expression tag

P, Q: 1 - 7 (UniProt: P21850)

PDB	External Database	Details
Dal 2	Ala 108	variant
Nh2 8	-	amidation

A, F: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Ala 203	Gly 203	conflict
Ser 326	Ala 326	conflict

B: 7 - 345 (UniProt: P54311)

PDB	External Database	Details
Met -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Gly 2	-	expression tag
Ser 3	-	expression tag
Leu 4	-	expression tag
Leu 5	-	expression tag
Gln 6	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	214340.3
Non-Polymers*	Number of molecules	17
	Total formula weight	5010.4
All*	Total formula weight	219350.7

*Water molecules are not included.