8F2P

Nef SF2 dimerization mutant bound to Hck SH3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	C-terminal core protein	polymer	149	17594.9	1	UniProt (P03407) Pfam (PF00469) In PDB	HIV-1 M:B_ARV2/SF2	Nef
2	B	Tyrosine-protein kinase HCK	polymer	73	8555.5	1	UniProt (P08631) Pfam (PF00018) In PDB	Homo sapiens (human)	Hematopoietic cell kinase,Hemopoietic cell kinase,p59-HCK/p60-HCK,p59Hck,p61Hck
3		water	water		18.0	4	Chemie (HOH)

Sequence modifications

A: 62 - 209 (UniProt: P03407)

PDB	External Database	Details
Met 61	-	initiating methionine
Asp 116	Leu 116	engineered mutation

B: 80 - 144 (UniProt: P08631)

PDB	External Database	Details
Met 79	-	initiating methionine
Leu 145	-	expression tag
Glu 146	-	expression tag
His 147	-	expression tag
His 148	-	expression tag
His 149	-	expression tag
His 150	-	expression tag
His 151	-	expression tag
His 152	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	26150.3
All*	Total formula weight	26150.3

*Water molecules are not included.