8EWY

Structure of Janus Kinase (JAK) dimer complexed with cytokine receptor intracellular domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Tyrosine-protein kinase	polymer	1173	136026.8	2	UniProt (B1ASP2) Pfam (PF18379) Pfam (PF18377) Pfam (PF17887) Pfam (PF21990) Pfam (PF07714) UniProt (by SIFTS) (P52332)	Mus musculus (house mouse)
2	C, D (C, D)	Interferon lambda receptor 1	polymer	85	9895.4	2	UniProt (Q8CGK5)	Mus musculus (house mouse)	IFN-lambda R1,Cytokine receptor class-II member 12,Cytokine receptor family 2 member 12,CRF2-12,Interleukin-28 receptor subunit alpha,IL-28 receptor subunit alpha,IL-28R-alpha,IL-28RA
3	E, G (A, B)	ADENOSINE	non-polymer		267.2	2	Chemie (ADN)
4	F, H (A, B)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)

Sequence modifications

A, B: 1 - 1153 (UniProt: B1ASP2)

PDB	External Database	Details
Phe 657	Val 657	engineered mutation
Gly 1154	-	expression tag
Ser 1155	-	expression tag
Asp 1156	-	expression tag
Arg 1157	-	expression tag
Lys 1158	-	expression tag
Ala 1159	-	expression tag
Ala 1160	-	expression tag
Val 1161	-	expression tag
Ser 1162	-	expression tag
His 1163	-	expression tag
Trp 1164	-	expression tag
Gln 1165	-	expression tag
His 1166	-	expression tag
His 1167	-	expression tag
His 1168	-	expression tag
His 1169	-	expression tag
His 1170	-	expression tag
His 1171	-	expression tag
His 1172	-	expression tag
His 1173	-	expression tag

C, D: 249 - 298 (UniProt: Q8CGK5)

PDB	External Database	Details
Gly 214	-	expression tag
Pro 215	-	expression tag
Arg 216	-	expression tag
Met 217	-	expression tag
Lys 218	-	expression tag
Gln 219	-	expression tag
Leu 220	-	expression tag
Glu 221	-	expression tag
Asp 222	-	expression tag
Lys 223	-	expression tag
Val 224	-	expression tag
Glu 225	-	expression tag
Glu 226	-	expression tag
Leu 227	-	expression tag
Leu 228	-	expression tag
Ser 229	-	expression tag
Lys 230	-	expression tag
Asn 231	-	expression tag
Tyr 232	-	expression tag
His 233	-	expression tag
Leu 234	-	expression tag
Glu 235	-	expression tag
Asn 236	-	expression tag
Glu 237	-	expression tag
Val 238	-	expression tag
Ala 239	-	expression tag
Arg 240	-	expression tag
Leu 241	-	expression tag
Lys 242	-	expression tag
Lys 243	-	expression tag
Leu 244	-	expression tag
Val 245	-	expression tag
Gly 246	-	expression tag
Glu 247	-	expression tag
Arg 248	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	291844.4
Non-Polymers*	Number of molecules	4
	Total formula weight	1388.9
All*	Total formula weight	293233.3

*Water molecules are not included.